Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

4.7K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
4.7K
X-ray Crystallography02:18

X-ray Crystallography

25.9K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
25.9K
Interference and Diffraction02:18

Interference and Diffraction

51.9K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
51.9K
Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

6.5K
Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
6.5K
Equation of Motion: General Plane motion01:22

Equation of Motion: General Plane motion

565
In the context of a rigid body's movement within a general plane, it is important to understand that this motion is typically triggered by external forces or couple moments exerted onto it. This principle can be explained through Newton's second law, which stipulates the translational motion of the body's center of mass along each axis.
Moreover, the body's center of mass experiences a rotational effect as a result of these couple moments. This rotation can be articulated as the...
565
Molecules and Compounds02:38

Molecules and Compounds

68.4K
Atoms and Molecules
68.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strain-Induced Density Fluctuations in Glassy Polymers.

Macromolecular rapid communications·2025
Same author

Suprapubic Open and Single-Port Laparoscopic Surgery for Urachal Remnant in Children: A Case Series.

Asian journal of endoscopic surgery·2025
Same author

Hypertrophic pyloric stenosis presenting with polyhydramnios in utero: A case report.

Pediatrics international : official journal of the Japan Pediatric Society·2025
Same author

Decortication with uniport video-assisted thoracoscopic surgery for empyema due to postoperative esophageal leakage: a report of two pediatric cases.

Surgical case reports·2024
Same author

Pre-operative Serum Albumin Predicts Native Liver Survival in Biliary Atresia.

African journal of paediatric surgery : AJPS·2024
Same author

Impact of donor and prolonged cold ischemia time of neonatal pig pancreas on neonatal pig islet transplant outcome.

Xenotransplantation·2020

Related Experiment Video

Updated: Jan 25, 2026

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
12:38

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

Published on: April 11, 2021

7.0K

Diffracted X-ray tracking method for recording single-molecule protein motions.

Hirofumi Shimizu1

  • 1Department of Integrative and Systems Physiology, Faculty of Medical Sciences, University of Fukui, Japan.

Biochimica Et Biophysica Acta. General Subjects
|May 12, 2019
PubMed
Summary

Diffracted X-ray tracking (DXT) reveals protein dynamics by monitoring gold nanocrystal probes. This method offers high temporal and spatial resolution, crucial for understanding protein function and drug discovery.

Keywords:
Diffracted X-ray trackingGold nanocrystalKcsA channelProtein dynamicsSingle-molecule

More Related Videos

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
16:21

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Published on: March 10, 2014

18.3K
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.8K

Related Experiment Videos

Last Updated: Jan 25, 2026

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
12:38

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies

Published on: April 11, 2021

7.0K
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking
16:21

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Published on: March 10, 2014

18.3K
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.8K

Area of Science:

  • Biophysics
  • Structural Biology
  • Biochemistry

Background:

  • Proteins undergo conformational changes essential for their function.
  • Static protein structures are abundant, but dynamic information during function is limited.
  • Diffracted X-ray tracking (DXT) offers a potential solution for capturing protein dynamics.

Purpose of the Study:

  • To review the effective application of DXT from a user's perspective.
  • To highlight the advantages and potential of DXT for studying protein dynamics.
  • To present an example using the KcsA channel and discuss recent developments.

Main Methods:

  • Attaching a gold nanocrystal to a target protein as a probe.
  • Monitoring X-ray diffraction spots from the gold nanocrystal to track protein motion.
  • Achieving high temporal (sub-millisecond) and spatial (0.1°) resolutions.

Main Results:

  • DXT successfully revealed intramolecular structural changes, such as conformational waves in the KcsA channel.
  • The method demonstrated high temporal and spatial resolutions for tracking molecular motions.
  • DXT enables simultaneous tracking of multiple molecules, beneficial for screening.

Conclusions:

  • DXT is a powerful technique for investigating protein conformational changes related to function.
  • Continuous development enhances DXT's applicability across various sample conditions.
  • DXT holds significant promise for drug discovery and understanding protein mechanisms.