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MiR-128-3p suppresses breast cancer cellular progression via targeting LIMK1

Junyong Zhao1, Dengfeng Li1, Lin Fang1

  • 1Department of Thyroid and Breast, Division of General Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, People's Republic of China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|May 12, 2019
PubMed

Insights

MicroRNAs (miRNAs) like miR-128-3p can suppress breast cancer growth and movement. High miR-128-3p levels in patients correlate with better breast cancer prognosis, suggesting therapeutic potential.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer is a leading global malignancy in women.
  • MicroRNAs (miRNAs) are key regulators of cellular processes, including cancer development.
  • Understanding miRNA roles offers insights into breast cancer mechanisms and treatment.

Purpose of the Study:

  • To investigate the function of miR-128-3p in breast cancer.
  • To identify target genes and pathways regulated by miR-128-3p.
  • To explore the prognostic value of miR-128-3p in breast cancer patients.

Main Methods:

  • Cell proliferation and motility assays were performed.
  • Cell cycle analysis was conducted to assess effects on cell cycle regulators (CDK4/CDK6/Cyclin D1, CDK2/Cyclin E1).
  • Target gene validation (LIMK1) and downstream effects (Cofilin 1) were confirmed. TCGA database analysis was used for clinical correlation.

Main Results:

  • miR-128-3p significantly suppressed breast cancer cell proliferation and motility.
  • Overexpression of miR-128-3p induced G0/G1 cell cycle arrest.
  • LIM domain kinase 1 (LIMK1) was identified as a direct target, leading to reduced LIMK1 and Cofilin 1 expression.
  • High miR-128-3p expression in patients correlated with better prognosis.

Conclusions:

  • miR-128-3p acts as a tumor suppressor in breast cancer.
  • The miR-128-3p/LIMK1/CFL1 pathway is crucial for regulating breast cancer progression.
  • miR-128-3p represents a potential therapeutic target and biomarker for breast cancer treatment.
Keywords:
Breast cancerCFL1LIMK1ProgressionmiR-128-3p

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