Related Experiment Video
Updated: Jan 25, 2026

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
Published on: September 13, 2021
Cryo-Electron Microscopy Methodology: Current Aspects and Future Directions
Radostin Danev1, Haruaki Yanagisawa1, Masahide Kikkawa1
1Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
Abstract:
Cryo-electron microscopy (cryo-EM) has emerged as a powerful structure determination technique. Its most prolific branch is single particle analysis (SPA), a method being used in a growing number of laboratories worldwide to determine high-resolution protein structures. Cryo-electron tomography (cryo-ET) is another powerful approach that enables visualization of protein complexes in their native cellular environment. Despite the wide-ranging success of cryo-EM, there are many methodological aspects that could be improved. Those include sample preparation, sample screening, data acquisition, image processing, and structure validation. Future developments will increase the reliability and throughput of the technique and reduce the cost and skill level barrier for its adoption.
Related Concept Videos
Cryo-electron Microscopy
Overview of Electron Microscopy
Scanning Electron Microscopy
Fundamental Principles
Accelerated...
Transmission Electron Microscopy
Immunogold Electron Microscopy
Preparation of Samples for Electron Microscopy

