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IFN-gamma is not an essential mediator of murine antibody responses in vitro
We have used purified murine gamma-interferon (Mu IFN-gamma) and anti-Mu IFN-gamma monoclonal antibody to study the participation of gamma-interferon (IFN-gamma) in in vitro plaque-forming cell (PFC) responses to antigen. We have studied several supernatants with T-cell replacing factor (TRF) activity as well as helper T-cell dependent anti-sheep red blood cells (SRBC) PFC responses. Our findings demonstrate that neither TRF- nor T-cell mediated responses are critically dependent upon IFN-gamma.
We have used purified murine gamma-interferon (Mu IFN-gamma) and anti-Mu IFN-gamma monoclonal antibody to study the participation of gamma-interferon (IFN-gamma) in in vitro plaque-forming cell (PFC) responses to antigen. We have studied several supernatants with T-cell replacing factor (TRF) activity as well as helper T-cell dependent anti-sheep red blood cells (SRBC) PFC responses. Our findings demonstrate that neither TRF- nor T-cell mediated responses are critically dependent upon IFN-gamma.