A HBT-based bifunctional fluorescent probe for the ratiometric detection of fluoride and sulphite in real samples

Based on a core of 2-(benzo[d]thiazol-2-yl)phenol (HBT), a bifunctional ratiometric fluorescent probe, HBT-FS, was constructed for the discriminative detection of fluoride (F^-) and sulphite (SO₃^2-) with high sensitivity and selectivity. HBT-FS itself displayed a green fluorescence with λ_Em^max = 498 nm. The treatment of HBT-FS with F^- resulted in a red fluorescence (λ_Em^max = 634 nm) with a large Stokes shift and a 291-fold enhancement in the ratio of the fluorescence intensity (I_{634 nm}/I_{498 nm}). Upon the addition of SO₃^2-, HBT-FS exhibited a blue fluorescence (λ_Em^max = 371 nm) and the ratiometric fluorescence enhancement was remarkable (9445 folds for I_{371 nm}/I_{498 nm}). HBT-FS was successfully used to qualitatively and quantitatively determine F^- and SO₃^2- in a ratiometric manner in real samples.