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Updated: Jan 25, 2026

A Protocol to Infect Caenorhabditis elegans with Salmonella typhimurium
Published on: June 26, 2014
Decrease of Salmonella typhimurium in Skim Milk and Egg by Heat and Ultrasonic Wave Treatment
Dorothy M Wrigley1, Natalie G Llorca1
1Division of Microbiology, Department of Biological Sciences, Mankato State University, Mankato, Minnesota 56002-8400.
Abstract:
Ultrasonic waves induce cavitation which is lethal for many bacteria. When Salmonella typhimurium was suspended in skim milk or brain heart infusion broth and placed in an ultrasonicating water bath, the number of bacteria decreased by 2 to 3 log CFU in a time dependent manner. The killing by ultrasonic waves was enhanced if the menstruum was simultaneously maintained at 50°C. Ultrasonic reduction in S. typhimurium numbers in liquid whole egg ranged from 1-3 log CFU at 50°C. The results indicate that indirect ultrasonic wave treatment is effective in killing Salmonella in some foods.
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