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Updated: Jan 25, 2026

Optical Clearing of Plant Tissues for Fluorescence Imaging
Published on: January 5, 2022
Massive volumetric imaging of cleared tissue: The necessary tools to be successful
Alan M Watson1, Simon C Watkins1
1The Department of Cell Biology and The Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA, USA.
Abstract:
Recent technological innovations in high-speed microscopes, tissue clearing methods, optics and computation have made it possible to image enormous biologic volumes. These techniques are applicable to a broad range of biomedical disciplines and are becoming a necessary tool for exploring biologic systems that extend beyond cellular microenvironments to entire organs and organisms. Here we broadly discuss the tool sets that are available for cleared tissue imaging and how various choices may influence the planning and feasibility of an experiment.
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