Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples
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Updated: Jan 25, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
Guinevere S M Lageveen-Kammeijer1, Noortje de Haan2, Pablo Mohaupt2
1Leiden University Medical Center, Center for Proteomics and Metabolomics, Postbus 9600, 2300 RC, Leiden, The Netherlands. g.s.m.kammeijer@lumc.nl.
Abstract:
The in-depth, high-sensitivity characterization of the glycome from complex biological samples, such as biofluids and tissues, is of utmost importance in basic biological research and biomarker discovery. Major challenges often arise from the vast structural diversity of glycans in combination with limited sample amounts. Here, we present a method for the highly sensitive characterization of released N-glycans by combining a capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) approach with linkage-specific derivatization of sialic acids and uniform cationic reducing end labelling of all glycans. This method allows the analysis of glycans at the attomole level, provides information on sialic acid isomers and enables the in-depth characterization of complex samples, even when available in minute amounts.
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