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Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
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Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples
Guinevere S M Lageveen-Kammeijer1, Noortje de Haan2, Pablo Mohaupt2
1Leiden University Medical Center, Center for Proteomics and Metabolomics, Postbus 9600, 2300 RC, Leiden, The Netherlands. g.s.m.kammeijer@lumc.nl.
Nature Communications
|May 16, 2019
Summary
This study introduces a highly sensitive method for characterizing N-glycans, even from small biological samples. The new technique enhances glycomic analysis for biological research and biomarker discovery.
Area of Science:
- Glycomics
- Biomarker Discovery
- Mass Spectrometry
Background:
- Characterizing the glycome is crucial for biological research and identifying biomarkers.
- Challenges include the diverse structures of glycans and limited sample availability.
Purpose of the Study:
- To develop a highly sensitive method for N-glycan characterization.
- To enable in-depth analysis of complex biological samples, even in minute quantities.
Main Methods:
- Combined capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS).
- Incorporated linkage-specific derivatization of sialic acids.
- Utilized uniform cationic reducing end labeling of all glycans.
Main Results:
- Achieved attomole-level analysis of glycans.
- Enabled differentiation of sialic acid isomers.
- Facilitated in-depth characterization of complex samples with limited amounts.
Conclusions:
- The presented method offers high sensitivity for N-glycan analysis.
- It overcomes challenges posed by sample limitations and glycan diversity.
- This technique advances glycomic research and biomarker discovery.
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