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Updated: Jan 25, 2026
05:52
Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals
Published on: October 20, 2019
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High-density multi-fiber photometry for studying large-scale brain circuit dynamics.
Yaroslav Sych1, Maria Chernysheva2,3, Lazar T Sumanovski2
1Brain Research Institute, University of Zurich, Zurich, Switzerland. sych@hifo.uzh.ch.
Nature Methods
|May 16, 2019
Summary
Researchers developed new high-density fiber arrays for simultaneously recording and perturbing neural activity across many brain regions in mice. This technology advances the study of large-scale brain dynamics during complex behaviors.
Area of Science:
- Neuroscience
- Systems Neuroscience
- Behavioral Neuroscience
Background:
- Understanding complex animal behavior requires assessing large-scale neural dynamics.
- Current techniques for monitoring brain-wide activity in behaving animals are limited.
Purpose of the Study:
- To introduce a novel high-density, chronically implantable optical fiber array system.
- To enable simultaneous multi-fiber photometry and optogenetic perturbations across numerous brain regions in mammals.
Main Methods:
- Development of compact, high-density optical fiber arrays for chronic implantation.
- Simultaneous calcium imaging (photometry) from 12-48 brain regions in mice performing a texture discrimination task.
- Optogenetic perturbation of specific brain regions using a spatial light modulator and VGAT-ChR2 mice.
Main Results:
- Successful simultaneous recordings from striatal, thalamic, hippocampal, and cortical areas during behavior.
- Demonstrated distributed network modulation and behavioral deficits upon perturbation of ventral thalamic nuclei.
- Validated multi-fiber photometry in freely moving mice, including simultaneous recordings during social interaction.
Conclusions:
- High-density multi-fiber arrays are versatile tools for investigating large-scale brain dynamics.
- This technology facilitates the study of neural circuits underlying complex behaviors.
- Enables simultaneous interrogation of neural activity and manipulation across distributed brain networks.
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