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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
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Multiplex genome editing of microorganisms using CRISPR-Cas.
Belén Adiego-Pérez1, Paola Randazzo2, Jean Marc Daran2
1Laboratory of Microbiology, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands.
FEMS Microbiology Letters
|May 16, 2019
Summary
CRISPR-Cas tools enable advanced genome editing in microbes for chemical production. This review covers multiplex editing and gene regulation strategies for industrial microorganisms.
Area of Science:
- Synthetic Biology
- Microbial Biotechnology
- Genome Engineering
Background:
- Microbial cell factories are crucial for chemical compound production.
- CRISPR-Cas nucleases have become essential tools for precise genome editing.
- Engineering microbes requires efficient and scalable genome modification techniques.
Purpose of the Study:
- To review the application of CRISPR-Cas tools in microbial genome editing.
- To highlight progress in multiplex genome editing for industrial bacteria and eukaryotes.
- To discuss CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) for gene regulation.
Main Methods:
- Review of CRISPR-Cas systems and their applications.
- Analysis of multiplex genome editing strategies.
- Evaluation of CRISPRi and CRISPRa for gene expression control.
Main Results:
- CRISPR-Cas tools facilitate multiplex genome editing in various microorganisms.
- CRISPRi and CRISPRa offer robust control over gene expression.
- Key factors for efficient multiplex genome editing have been identified.
Conclusions:
- CRISPR-Cas technology significantly advances microbial engineering for chemical synthesis.
- Future developments promise expanded applications in synthetic biology and industrial biotechnology.
- Optimized multiplex genome editing is vital for creating efficient microbial cell factories.
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