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Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope

Wenyue Liu1,2,3, Fan Zheng1,2,3, Yucai Wang4

  • 1Division of Molecular and Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, China.

Journal of Molecular Cell Biology
|May 16, 2019
PubMed

Insights

Alp7, Alp14, and Mto1 proteins coordinate microtubule nucleation at the nuclear envelope (NE). This study reveals their interdependent localization and function in promoting microtubule regrowth from noncentrosomal microtubule-organizing centers (MTOCs).

Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Molecular Cell Biology

Background:

  • Microtubules organize cellular processes and are nucleated from centrosomes and noncentrosomal microtubule-organizing centers (MTOCs), including the nuclear envelope (NE).
  • Proteins like Mto1/CDK5RAP2 and Alp14/TOG/XMAP215 are known microtubule nucleation factors, but their recruitment and coordination at noncentrosomal MTOCs remain unclear.

Purpose of the Study:

  • To investigate the roles and coordination of Alp7/TACC, Alp14/TOG/XMAP215, and Mto1/CDK5RAP2 in interphase microtubule nucleation at the NE in fission yeast.
  • To elucidate the mechanisms governing the localization and function of these proteins at noncentrosomal MTOCs.

Main Methods:

  • Utilized fission yeast (Schizosaccharomyces pombe) as a model system.
  • Observed microtubule regrowth dynamics following depolymerization.
  • Investigated protein localization at the NE using microscopy.
  • Performed genetic manipulations to assess protein dependency and functional rescue.

Main Results:

  • Efficient microtubule regrowth from the NE requires Alp7, Alp14, and Mto1.
  • Alp7 and Mto1 exhibit interdependent localization to the NE.
  • Alp14 localization to the NE is dependent on Alp7 and Mto1.
  • Tethering Mto1 to the NE partially rescues microtubule organization in Alp7-deficient cells.

Conclusions:

  • Alp7, Alp14, and Mto1 function concertedly to regulate interphase microtubule regrowth from the NE.
  • This study defines a novel mechanism for coordinating microtubule nucleation at a key noncentrosomal MTOC.
Keywords:
fission yeastmicrotubulemicrotubule nucleationmicrotubule-associated proteinnuclear envelope

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