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Updated: Jan 24, 2026

Mouse Epidermal Neural Crest Stem Cell EPI-NCSC Cultures
Published on: May 9, 2008
Competitive Repopulation Assay of Long-Term Epidermal Stem Cell Regeneration Potential
Carmen Segrelles1,2,3, Karla Santos-de-Frutos1,2,3, Jesús M Paramio1,2,3
1Molecular Oncology Unit, CIEMAT (ed 70A), Madrid, Spain.
Abstract:
Epidermal stem cells are responsible for normal tissue homeostasis and contribute to tissue regeneration during injury. Several assays measuring stem cell frequency and function can be used to assess epidermal stem cell potential. However, the ultimate assay that accounts for stemness is the capacity to sustain in vivo long-term tissue regeneration and maintenance. We can use this type of analysis to interrogate whether a specific genetic alteration (e.g., activation or inactivation of any gene thought to be involved in stem cell quiescence or proliferation) confers increased or decreased stem cell potential.
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