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Updated: Jan 24, 2026

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
Published on: July 24, 2020
Simple and robust calibration procedure for k-linearization and dispersion compensation in optical coherence
Xavier Attendu1,2, Roosje M Ruis1, Caroline Boudoux2
1University of Amsterdam, Amsterdam University Medical Center, Department of Biomedical Engineering a, The Netherlands.
A new calibration method for Fourier-domain optical coherence tomography (FD-OCT) simplifies system adjustments. This robust procedure uses minimal measurements for accurate dispersion compensation and signal sampling, enhancing axial resolution.
Area of Science:
- Biomedical Optics
- Medical Imaging
- Optical Engineering
Background:
- Achieving optimal axial resolution in Fourier-domain optical coherence tomography (FD-OCT) relies on precise signal sampling and dispersion compensation.
- Traditional calibration methods for these parameters in FD-OCT often involve complex protocols and require prior system knowledge.
- Existing calibration techniques can be time-consuming and may necessitate specialized hardware, limiting their widespread application.
Purpose of the Study:
- To introduce a simplified and highly robust calibration procedure for FD-OCT systems.
- To enable simultaneous determination of correction vectors for nonlinear wavenumber sampling and dispersion compensation.
- To provide a method that requires minimal system information and simple measurements.
Main Methods:
- The proposed method utilizes only two simple mirror measurements.
- It does not require prior knowledge of the illumination source or detection scheme.
- The procedure is implemented through numerical signal processing for calibration.
Main Results:
- The method successfully determines correction vectors for both nonlinear wavenumber sampling and dispersion compensation simultaneously.
- It demonstrates applicability to both spectral domain OCT and swept-source OCT systems.
- The procedure proved effective and robust in experimental validations on both OCT types.
Conclusions:
- A simplified, robust, and broadly applicable calibration method for FD-OCT has been developed.
- This technique reduces calibration complexity and hardware requirements, enhancing accessibility.
- The method can serve as a cost-effective validation tool for essential FD-OCT calibration hardware.
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