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Updated: Jan 24, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

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miRNA deregulation targets specific pathways in leiomyosarcoma development: an in silico analysis

Clara Benna1,2, Senthilkumar Rajendran3, Marco Rastrelli4

  • 1Department of Surgery Oncology and Gastroenterology, University of Padova, Padua, Italy. clara.benna@unipd.it.

Journal of Translational Medicine
|May 16, 2019
PubMed
Abstract

Insights

Differential microRNA (miRNA) expression in leiomyosarcoma suggests roles for RNA Polymerase III, tRNA functions, and dopamine signaling in cancer development. This study highlights data mining for rare tumor research.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) regulate gene expression and are implicated in human diseases, including cancer.
  • Soft tissue sarcomas, like leiomyosarcoma, are rare malignancies with unclear cancerogenesis.
  • Leiomyosarcoma, a common subtype, originates from smooth muscle tissue.

Purpose of the Study:

  • To investigate the role of differential microRNA (miRNA) expression in leiomyosarcoma development.
  • To identify specific miRNAs and their target genes involved in leiomyosarcoma pathogenesis.
  • To explore molecular pathways associated with leiomyosarcoma through bioinformatics analysis.

Main Methods:

  • Utilized the Sarcoma microRNA Expression Database to identify differentially expressed miRNAs between leiomyosarcoma and smooth muscle samples.
  • Employed TargetScan and Enrichr tools to predict miRNA targets and analyze enriched molecular pathways.
  • Conducted protein-protein network analysis using the STRING web tool.

Main Results:

  • Identified 301 statistically significant differentially expressed miRNAs between leiomyosarcoma and smooth muscle samples.
  • Predicted 438 high-confidence target genes for 172 miRNAs.
  • Pathway analysis implicated RNA Polymerase III, tRNA functions, and dopamine-mediated synaptic neurotransmission in leiomyosarcoma development.

Conclusions:

  • Data mining of public repositories effectively suggests molecular pathways in rare tumor pathogenesis.
  • Identified potential miRNA involvement in leiomyosarcoma development.
  • Findings provide a foundation for further research into leiomyosarcoma molecular mechanisms.
Keywords:
DopamineLeiomyosarcomaPathway analysisRNA pol IIIRNA polymerase IIISarcomamiRNAmicroRNA

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