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Junctional tumor suppressors interact with 14-3-3 proteins to control planar spindle alignment

Yu-Ichiro Nakajima1,2,3, Zachary T Lee4, Sean A McKinney4

  • 1Stowers Institute for Medical Research, Kansas City, MO yuichiro.nakajima.d2@tohoku.ac.jp.

The Journal of Cell Biology
|May 16, 2019
PubMed

Insights

Planar spindle orientation in Drosophila epithelia relies on Scribbled (Scrib) and Discs large (Dlg) tumor suppressors. These proteins, along with 14-3-3 and Mud proteins, are crucial for maintaining tissue architecture during cell division.

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Genetics

Background:

  • Proper mitotic spindle orientation is vital for tissue development and homeostasis.
  • Planar spindle alignment maintains epithelial architecture, while misorientation disrupts it.
  • In vivo mechanisms regulating planar spindle orientation are not fully understood.

Purpose of the Study:

  • To investigate the in vivo mechanisms controlling planar spindle orientation in the Drosophila wing disc epithelium.
  • To identify key proteins involved in regulating spindle positioning during epithelial cell division.

Main Methods:

  • Genetic analysis in Drosophila wing discs.
  • Co-immunoprecipitation and mass spectrometry to identify protein interactions.
  • Analysis of protein localization and mitotic spindle movements.

Main Results:

  • Scribbled (Scrib) and Discs large (Dlg) tumor suppressors are essential for planar spindle orientation.
  • Scrib is required for Dlg's junctional localization, and both influence spindle movements.
  • 14-3-3 proteins interact with Dlg and are crucial for spindle orientation and epithelial integrity.

Conclusions:

  • 14-3-3 proteins function with Scrib, Dlg, and Mud to regulate planar cell division.
  • Loss of 14-3-3 proteins leads to abnormal spindle orientation, basal cell delamination, and apoptosis.
  • These findings elucidate a novel pathway controlling spindle orientation in epithelial tissues.

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