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Updated: Jan 24, 2026
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
Published on: November 21, 2010
Unsupervised determination of protein crystal structures
Ivan S Ufimtsev1, Michael Levitt1
1Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305 ufimtsev@stanford.edu michael.levitt@stanford.edu.
Abstract:
We present a method for automatic solution of protein crystal structures. The method proceeds with a single initial model obtained, for instance, by molecular replacement (MR). If a good-quality search model is not available, as often is the case with MR of distant homologs, our method first can automatically screen a large pool of poorly placed models and single out promising candidates for further processing if there are any. We demonstrate its utility by solving a set of synthetic cases in the 2.9- to 3.45-Å resolution.
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