Related Experiment Video
Updated: Jan 24, 2026

Rabies Necropsy Techniques in Large and Small Animals
Published on: July 30, 2019
Molecular epidemiological analysis of wild animal rabies isolates from India
Gundallhalli Bayyappa Manjunatha Reddy1, Rajendra Singh2, Karam Pal Singh2
1Department of Veterinary Pathology, ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India.
Aim:
This study was conducted to know the genetic variability of rabies viruses (RVs) from wild animals in India.
Materials And Methods:
A total of 20 rabies suspected brain samples of wild animals from different states of India were included in the study. The samples were subjected for direct fluorescent antibody test (dFAT), reverse transcription polymerase chain reaction (RT-PCR), and quantitative reverse transcriptase real-time PCR (RT-qPCR). The phylogenetic analysis of partial nucleoprotein gene sequences was performed.
Results:
Of 20 samples, 11, 10, and 12 cases were found positive by dFAT, RT-PCR, and RT-qPCR, respectively. Phylogenetic analysis showed that all Indian wild RVs isolates belonged to classical genotype 1 of Lyssavirus and were closely related to Arctic/Arctic-like single cluster indicating the possibility of a spillover of rabies among different species.
Conclusion:
The results indicated the circulation of similar RVs in sylvatic and urban cycles in India. However, understanding the role of wild animals as reservoir host needs to be studied in India.
More Related Videos
07:40Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications
Published on: June 29, 2020
07:54Author Spotlight: Rabies-Specific Antibody Isotypes Detection in Sera or Cerebral Spinal Fluid Using an IFA Test
Published on: January 19, 2024
Related Concept Videos
Introduction to Epidemiology
Causality in Epidemiology
Study Designs in Epidemiology
Observational studies are those where the researcher does not intervene but rather observes natural variations. They include cross-sectional, cohort, and...
Confounding in Epidemiological Studies
Bias in Epidemiological Studies
Statistical Methods for Analyzing Epidemiological Data