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Sensitization of Resistance Ovarian Cancer Cells to Cisplatin by Biogenic Synthesized Silver Nanoparticles through

Tayebe Ramezani1, Mohamad Nabiuni1, Javad Baharara2

  • 1Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.

Iranian Journal of Pharmaceutical Research : IJPR
|May 16, 2019
PubMed

Insights

Curcumin-coated silver nanoparticles (cAgNPs) combined with cisplatin restore sensitivity in drug-resistant ovarian cancer cells. This synergistic approach enhances apoptosis and upregulates p53, offering a promising strategy against cisplatin resistance.

Area of Science:

  • Nanomedicine
  • Cancer Therapeutics
  • Drug Resistance

Background:

  • Drug resistance is a major challenge in cancer treatment, necessitating novel therapeutic strategies.
  • Silver nanoparticles (AgNPs) exhibit anticancer properties and are explored for combination therapies.
  • Curcumin-coated silver nanoparticles (cAgNPs) offer potential synergistic effects with conventional chemotherapeutics.

Purpose of the Study:

  • To investigate the synergistic effects of biogenic curcumin-coated silver nanoparticles (cAgNPs) and cisplatin on cisplatin-resistant ovarian cancer cells (A2780).
  • To evaluate the ability of cAgNPs to restore sensitivity to cisplatin in resistant ovarian cancer models.
  • To assess the impact of combined therapy on apoptosis induction and gene expression.

Main Methods:

  • Cell viability was assessed using MTT assay.
  • Apoptosis was evaluated through Acridine orange/propidium iodide (AO/PI), DAPI staining, and Annexin V/PI assays.
  • Caspase 3/9 activation and gene expression (p53, MMP-9) via RT-PCR were analyzed.

Main Results:

  • Combined treatment with non-toxic concentrations of cAgNPs (2 µg/mL) and cisplatin (2.5 µg/mL) reduced proliferation by approximately 50%.
  • Synergistic use significantly increased apoptosis induction, as evidenced by AO/PI, DAPI, and Annexin V/PI assays.
  • Combined therapy led to increased caspase 3/9 activity, upregulated p53 expression, and downregulated MMP-9 expression.

Conclusions:

  • Combination of cAgNPs and cisplatin effectively overcomes cisplatin resistance in ovarian cancer cells.
  • The synergistic approach enhances apoptosis through caspase activation and modulation of p53 and MMP-9.
  • This combination therapy presents a promising strategy to improve treatment outcomes for cisplatin-resistant ovarian cancer.
Keywords:
CisplatinCurcuminNanoparticlesResistanceSilver

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