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Updated: Jan 24, 2026
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Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Published on: March 15, 2019
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[From panel diagnostics to comprehensive genomic analysis : Infobesity or empowerment?]
J Leichsenring1, D Kazdal1, C Ploeger1
1Pathologisches Institut, Molekularpathologisches Zentrum, Universitätsklinikum Heidelberg, Heidelberg, Deutschland.
Der Pathologe
|May 16, 2019
Summary
Precision oncology uses molecular targets for targeted therapy, often identified by next-generation sequencing (NGS). Holistic genomic approaches like whole-genome sequencing offer comprehensive insights for complex cancer cases.
Area of Science:
- Oncology
- Genomics
- Molecular Biology
Background:
- Precision oncology is central to modern cancer therapy.
- Targeted therapy relies on identifying specific molecular targets.
- Next-generation sequencing (NGS) is a primary tool for this identification.
Purpose of the Study:
- To summarize and discuss key aspects and applications of genomic analysis methods.
- To highlight the role of NGS and more holistic approaches in precision oncology.
Main Methods:
- Next-generation sequencing (NGS) for identifying biomarkers, gene fusions, and copy number alterations.
- Whole-genome sequencing (WGS) and whole-exome sequencing (WES) for comprehensive genomic analysis.
- Transcriptome analysis for a holistic view of gene expression.
Main Results:
- NGS panels can identify approved predictive biomarkers and molecular targets.
- Panel design and method determine the scope of detectable alterations.
- Holistic genomic approaches (WGS, WES, transcriptome) are necessary for complex clinical scenarios.
Conclusions:
- Various genomic methods, including NGS and holistic approaches, are crucial in precision oncology.
- The choice of method depends on the clinical scenario and required comprehensiveness.
- Integration into clinical trials is essential for advanced genomic analyses.
Keywords:
DNA copy number variationsDNA mutational analysisGene expression profilingHigh-throughput nucleotide sequencingWhole genome sequencingMore Related Videos
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