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Related Experiment Videos

Cell surface changes in capping studied by correlated fluorescence and scanning electron microscopy.

M J Karnovsky, E R Unanue

    Laboratory Investigation; a Journal of Technical Methods and Pathology
    |December 1, 1978
    PubMed
    Summary

    Researchers developed a method to observe the same cell using fluorescence and scanning electron microscopy. This revealed cell surface changes during antibody-induced B lymphocyte capping, linking these changes to cell translocation, not capping itself.

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    Proceedings of the National Academy of Sciences of the United States of America·2000

    Area of Science:

    • Immunology
    • Cell Biology
    • Microscopy

    Background:

    • B lymphocytes display immunoglobulin (Ig) on their surfaces, crucial for immune response.
    • Antibody-cell interactions trigger dynamic surface rearrangements.

    Purpose of the Study:

    • To correlate fluorescence microscopy and scanning electron microscopy for observing identical cells.
    • To investigate sequential cell surface changes in mouse B lymphocytes upon interaction with anti-Ig antibodies.

    Main Methods:

    • Developed a correlative microscopy technique combining fluorescence and scanning electron microscopy.
    • Observed mouse B lymphocytes interacting with fluoresceinated anti-Ig antibodies.

    Main Results:

    • Observed rapid cell surface changes including diffuse staining, patching, and capping.

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  • Documented microvilli disappearance, ruffle/lamellipodia formation, and cell translocation opposite the cap.
  • Identified that T-cells undergoing translocation exhibit similar surface changes as B-cells.

    • Conclusions:

    • Cell surface changes are not solely due to patching and capping.
    • These dynamic surface alterations are an expression of antibody-induced cell translocation.
    • The contractile apparatus underlies these observed cellular phenomena.