Related Experiment Video

Updated: Jan 24, 2026

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function
06:14

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

Published on: June 11, 2017

8.8K

Expression of (pro)renin receptor and its effect on endothelial cell proliferation in infantile hemangioma

Bede van Schaijik1, Swee T Tan2,3, Reginald W Marsh1,4

  • 1Gillies McIndoe Research Institute, Wellington, New Zealand.

Pediatric Research
|May 16, 2019
PubMed

Insights

Renin activates the (pro)renin receptor (PRR) to promote infantile hemangioma (IH) cell proliferation via the wnt signaling pathway. This finding offers a new therapeutic target for IH treatment.

Area of Science:

  • Vascular Biology
  • Oncology
  • Endocrinology

Background:

  • Infantile hemangioma (IH) proliferation is linked to the renin-angiotensin system (RAS).
  • The (pro)renin receptor (PRR) is a key RAS component involved in wnt signaling.
  • PRR activation by renin is hypothesized to drive IH growth.

Purpose of the Study:

  • To investigate the expression and role of PRR in infantile hemangioma.
  • To determine if renin directly stimulates IH cell proliferation.
  • To elucidate the signaling pathway involved in renin-induced IH growth.

Main Methods:

  • Immunohistochemical (IHC) staining and NanoString analysis to assess PRR expression in IH tissues and cells.
  • MTT assay to evaluate the effect of renin on IH cell viability.
  • RT-qPCR to analyze stem cell gene expression.
  • Utilized wnt receptor blocker dickkopf-1 to assess pathway involvement.

Main Results:

  • PRR was confirmed to be expressed at both transcriptional and translational levels in IH endothelial and non-endothelial cells.
  • Renin administration significantly increased the number of viable IH cells.
  • The proliferative effect of renin was blocked by dickkopf-1, indicating wnt pathway activation.

Conclusions:

  • Renin-induced PRR activation promotes IH cell proliferation through the wnt signaling pathway.
  • This mechanism may explain cell accumulation during the proliferative phase of IH.
  • Targeting the renin-PRR-wnt axis presents a potential therapeutic strategy for problematic IH.
Abstract

More Related Videos

Quantitation of Endothelial Cell Adhesiveness In Vitro10:24

Quantitation of Endothelial Cell Adhesiveness In Vitro

Published on: June 18, 2015

11.9K
Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells04:55

Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells

Published on: February 23, 2011

11.3K

Related Concept Videos

Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.0K
Internal Receptors01:31

Internal Receptors

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
74.3K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.1K
Antihypertensive Drugs: Direct Renin Inhibitors01:25

Antihypertensive Drugs: Direct Renin Inhibitors

The renin-angiotensin-aldosterone system (RAAS) is an intricate physiological pathway involving numerous enzymes and hormones, including renin, angiotensin-converting enzyme (ACE), angiotensin I and II, and aldosterone. Imbalances within this system increase the production of angiotensin II and aldosterone. Increased angiotensin II levels promote vasoconstriction and blood pressure elevation. Concurrently, higher aldosterone levels stimulate sodium and water reabsorption in the kidneys,...
1.4K
Types of Receptors: Cell Surface Receptors01:28

Types of Receptors: Cell Surface Receptors

Cell-surface receptors, also known as transmembrane receptors, are cell surface, membrane-anchored (integral) proteins that bind to external ligand molecules. This type of receptor spans the plasma membrane and performs signal transduction, converting an extracellular signal into an intracellular signal. Ligands that interact with cell-surface receptors do not have to enter the cell that they affect. Cell-surface receptors are also called cell-specific proteins or markers because they are...
26.9K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.3K