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Lipid Vesicle-Coated Complex Coacervates.

Fatma Pir Cakmak1, Alex T Grigas1, Christine D Keating1

  • 1Department of Chemistry , Pennsylvania State University , University Park , Pennsylvania 16802 , United States.

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|May 17, 2019
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Complex coacervates and phospholipid vesicles interact, forming distinct structures. Polyelectrolyte properties influence vesicle distribution and biomolecule diffusion, crucial for applications like bioreactors.

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Area of Science:

  • Physical Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Complex coacervates are vital for compartmentalization in diverse fields.
  • Lipid vesicles (self-assembled lipid structures) are common in biological and commercial systems.
  • Understanding vesicle-coacervate interactions is key to controlling these systems.

Purpose of the Study:

  • To systematically investigate the interactions between anionic phospholipid vesicles and various complex coacervates.
  • To determine how coacervate composition and properties affect vesicle behavior.
  • To assess the impact of vesicle-coacervate interactions on biomolecule diffusion.

Main Methods:

  • Anionic phospholipid vesicles were introduced to coacervate systems formed from different polycations and polyanions.
  • Vesicle distribution (interface assembly, aggregation) was observed.
  • Factors like charge density and charge ratio were analyzed.
  • RNA oligonucleotide uptake was measured.

Main Results:

  • Vesicles assembled at the coacervate interface and/or formed aggregates.
  • Polyelectrolyte charge density and charge ratio significantly impacted vesicle distribution.
  • Interactions in the supernatant phase influenced pre-assembly aggregation.
  • Vesicle coronas affected RNA diffusion into coacervate droplets.

Conclusions:

  • Vesicle behavior in coacervates is tunable by adjusting polyelectrolyte properties.
  • Controlling vesicle-coacervate interactions can modulate biomolecule diffusion.
  • Systems with uniform vesicle coronas that permit biomolecule entry are promising for bioreactor applications.