Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
GIS Software, Hardware, and Sources of GIS Data01:23

GIS Software, Hardware, and Sources of GIS Data

774
A Geographic Information System (GIS) combines specialized software and hardware to effectively manage, analyze, and present spatial and related data. GIS software includes critical functionalities such as a user interface for easy navigation, database management tools for handling spatial and attribute data, and data retrieval features for efficient access. Analytical tools transform raw data into insights, while display functions produce maps and reports in various formats for effective...
774
Computed Tomography01:10

Computed Tomography

8.1K
Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
8.1K
Design Example: Traverse Angle Computations01:25

Design Example: Traverse Angle Computations

317
Traverse angle computations are a critical component of surveying, used to compute the internal angles within a closed traverse. A traverse consists of a series of connected lines forming a closed loop, often used for land boundary delineation or mapping. Calculating the internal angles ensures accuracy in the traverse geometry and is essential for checking survey data integrity.The process begins with known azimuths and bearings of the traverse sides. Internal angles at each vertex are...
317
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

15.4K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
15.4K
Electric Field01:16

Electric Field

12.3K
Consider two point charges, each exerting Coulomb force on the other. It is possible to describe the Coulomb interaction via an intermediate step by defining a new physical quantity called the electric field.
In the new picture, imagine that the first charge sets up an electric field independent of all other charges in the universe. When another charge comes in its vicinity, the second charge experiences an electric force depending on the electric field at that point. The source charge does not...
12.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

Nature methods·2026
Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

bioRxiv : the preprint server for biology·2026
Same author

Denoising framework for X-ray absorption spectroscopy data.

Journal of synchrotron radiation·2026
Same author

Simultaneous modeling of water-leaving reflectance and sun and sky glint contributions from imaging spectroscopy measurements.

Applied optics·2026
Same author

Nondestructive X-ray tomography of brain tissue ultrastructure.

Nature methods·2025
Same author

Pan-cortical cellular imaging in freely behaving mice using a miniaturized micro-camera array microscope (mini-MCAM).

Science advances·2025
Same journal

Correction: A method for supervoxel-wise association studies of age and other non-imaging variables from coronary computed tomography angiograms.

Scientific reports·2026
Same journal

Poly(bromophenol blue)/CoSn(OH)<sub>6</sub> cubic particles modified pencil graphite electrode for electrochemical determination of diphenhydramine.

Scientific reports·2026
Same journal

Dietary Chlorella, Spirulina, and acidifier modulate jejunal cytokine-related gene expression in broiler chickens.

Scientific reports·2026
Same journal

Perceived physical activity barriers in university students: associations with fatigue and eating behaviours.

Scientific reports·2026
Same journal

Refuge limitation structures habitat use in agricultural landscapes: evidence from Sunda pangolins.

Scientific reports·2026
Same journal

Lightweight stateless transaction verification with outsourced witness updates for UTXO blockchains.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Jan 24, 2026

Long-term Imaging Mammalian Cells using Wide-Field Microscopy
15:00

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Published on: November 30, 2006

5.5K

Low-cost, sub-micron resolution, wide-field computational microscopy using opensource hardware.

Tomas Aidukas1, Regina Eckert2, Andrew R Harvey3

  • 1Imaging Concepts Group, School of Physics and Astronomy, University of Glasgow, Scotland, G12 8QQ, UK.

Scientific Reports
|May 17, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a low-cost, Raspberry Pi-based microscope using Fourier ptychography (FP). It achieves high-resolution, 25-megapixel images, making advanced biomedical imaging more accessible.

More Related Videos

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
09:49

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

Published on: October 8, 2013

17.1K
Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
06:42

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

24.1K

Related Experiment Videos

Last Updated: Jan 24, 2026

Long-term Imaging Mammalian Cells using Wide-Field Microscopy
15:00

Long-term Imaging Mammalian Cells using Wide-Field Microscopy

Published on: November 30, 2006

5.5K
Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
09:49

Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

Published on: October 8, 2013

17.1K
Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone
06:42

Wide-field Fluorescent Microscopy and Fluorescent Imaging Flow Cytometry on a Cell-phone

Published on: April 11, 2013

24.1K

Area of Science:

  • Biomedical Imaging
  • Computational Microscopy
  • Optical Engineering

Background:

  • Conventional low-cost microscopy is limited by field of view and resolution.
  • Advancements in consumer photography and computation offer opportunities for cost reduction in biomedical imaging.

Purpose of the Study:

  • To develop a disruptive, low-cost microscopy technique.
  • To achieve high-resolution and large field-of-view imaging using affordable components.

Main Methods:

  • Utilized a Raspberry Pi single-board computer and color camera.
  • Implemented Fourier ptychography (FP) for computational image reconstruction.
  • Developed novel image-construction techniques to address limitations of low-cost sensors, aberrated lenses, and 3D-printed structures.

Main Results:

  • Successfully constructed 25-megapixel images with sub-micron resolution.
  • Overcame challenges associated with low-cost Bayer color sensors and optical aberrations.
  • Demonstrated a high performance-to-cost ratio for the microscopy system.

Conclusions:

  • The developed Fourier ptychography microscopy technique offers a cost-effective solution for high-throughput biomedical imaging.
  • This technology has potential applications in drug discovery, digital pathology, and global health screening.
  • Open-source availability of designs promotes accessibility and further development.