Immunological relationship among glycogen-protein complexes of different species

Glycogen-protein complexes from the muscles of Celerio euphorbiae and horse as well as from the livers of frog, carp and man were isolated and used as antigens. After immunization of rabbits antisera were obtained from which fractions of immunoglobulins were isolated. Antibodies were used in agar immunodiffusion tests and in quantitative reaction of immunoprecipitation with glycogens. The immunoprecipitation test proved the occurrence of quantitative differences in cross reaction of complexes with different origin antibodies, testifying to variability of their antigen properties. The immunodiffusion test allows to suppose the presence in antigens of at least two determinants of protein-carbohydratic character.