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Does butein affect adipogenesis?

Bianca Hemmeryckx1, Christine Vranckx1, Dries Bauters1

  • 1a Department Cardiovascular Sciences , Center for Molecular and Vascular Biology, KU Leuven , Leuven , Belgium.

Adipocyte
|May 17, 2019
PubMed

Insights

This study found that butein, a plant flavonoid, did not significantly affect preadipocyte differentiation or adipose tissue browning in mice. Butein did not alter ADAMTS5 (A Disintegrin And Metalloproteinase with ThromboSpondin motifs 5) or UCP-1 (uncoupling protein-1) gene expression in these processes.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Metabolic Research

Background:

  • Butein, a plant flavonoid chalcone, is suggested to possess anti-adipogenic properties.
  • Previous studies indicated butein might impair preadipocyte differentiation, limit adipose tissue development, and promote white adipose tissue browning in rodents.

Purpose of the Study:

  • To investigate if butein's potential anti-adipogenic effects are mediated by the reduction of ADAMTS5 (A Disintegrin And Metalloproteinase with ThromboSpondin motifs 5) expression.
  • To evaluate butein's impact on preadipocyte differentiation and adipose tissue browning in vitro and in vivo.

Main Methods:

  • Murine 3T3-L1 and 3T3-F442A preadipocytes were differentiated with butein, and gene expression of Adamts5 and Ucp-1 was analyzed.
  • ThermoMice and C57BL6/Rj mice were administered butein, followed by analysis of adipose tissue for Adamts5 and Ucp-1 mRNA levels.
  • Oil Red O staining and differentiation marker analysis were performed to assess adipogenesis.

Main Results:

  • Butein did not affect Adamts5 or Ucp-1 expression during preadipocyte differentiation in vitro.
  • No significant impact of butein on the extent of adipocyte differentiation was observed.
  • In vivo administration of butein to mice did not enhance Adamts5 or Ucp-1 expression in adipose tissue.

Conclusions:

  • The study did not demonstrate significant effects of butein on preadipocyte differentiation or adipose tissue development and browning.
  • Butein did not alter the expression of Adamts5 or Ucp-1 during these processes in the tested models.
Keywords:
ADAMTS5Buteinadipocyte differentiationadipogenesisbrowning

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