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Hyaluronidase Pretreatment Improves the Cryopreservation of Human Ovarian Tissue
Z Xiao1, Z Y Huang1, Y Y Zhang1
1Reproductive Medical Center of West China 2nd University Hospital, Key Laboratory of Birth Defects and Related Diseases of women and Children, Ministry of Education, Sichuan University, Chengdu 610041, China.
Background:
Slow freezing has been widely applied to preserve human ovarian tissue.
Objective:
The aim of this study is to determine whether human ovarian cortex pretreated with hyaluronidase can improve the protective effect of cryopreservation.
Materials And Methods:
Human ovarian biopsies from seven patients were cryopreserved using slow freezing. The samples in the experimental group were incubated in culture medium with hyaluronidase for a short time before cryopreservation. The histology of the thawed ovarian tissue was investigated; the levels of steroid hormone in the culture media were then used to evaluate the development and function of thawed ovarian tissue.
Results:
The result showed that the percentage of morphologically abnormal primordial follicles was higher in the freezing control than in the experimental group (P<0.05). 17β-estradiol (E2) and progesterone (P4) concentrations were significantly higher in the experimental group than in the freezing control group (P<0.05).
Conclusion:
Our study showed that human ovarian cortex pretreatment with hyaluronidase can improve the protective effect of cryopreservation by improving the penetration of cryoprotectant.
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