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Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
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MALDI-TOF MS Spectral Variation Is Observed between Fungal Samples Grown under Identical Conditions after Long-term
M A Reeve1, H Stewart2, M J Ryan2
1CABI, Bakeham Lane, Egham, Surrey, TW20 9TY, UK. M.Reeve@cabi.org.
Cryo Letters
|May 17, 2019
Summary
Preservation methods significantly impact fungal spectra analyzed by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). Different storage conditions, including cryopreservation and freeze-drying, lead to distinct spectral profiles, affecting fungal identification.
Area of Science:
- Microbiology
- Analytical Chemistry
- Mycology
Background:
- Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a valuable technique for protein analysis.
- Filamentous fungi present challenges in MALDI-TOF MS due to spectral variability influenced by growth conditions and time.
Purpose of the Study:
- To investigate how different preservation methods affect MALDI-TOF MS spectra of filamentous fungi.
- To determine if fungal preservation conditions introduce additional variance in spectral data.
Main Methods:
- Investigated Fusarium nygamai IMI 383003.
- Compared spectral data from fungi preserved by liquid nitrogen cryopreservation, freeze-drying, and storage under oil.
Main Results:
- Significant differences in MALDI-TOF MS spectra were observed based on preservation methods.
- High reproducibility was maintained between replicate sample preparations and culturing plates.
Conclusions:
- Fungal preservation techniques, including storage and cryopreservation, demonstrably alter MALDI-TOF MS spectra.
- These variations must be considered for accurate fungal identification using MALDI-TOF MS.
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