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Human Gametes Cryopreservation with Cryoprotectant Modified by Egg Yolk
A Grigorieva1, E Simonenko2, S Garmaeva1
1Lomonosov Moscow State University, Physical Department, GSP-1, Leninskie gory, Moscow, Russia.
Egg yolk enhances cryoprotectants for improved cell survival. Custom modifications with egg yolk and lecithin show higher cell viability, motility, and membrane integrity compared to commercial options.
Area of Science:
- Reproductive biology
- Cellular cryopreservation
Background:
- Egg yolk modification is a proven strategy to enhance cryoprotectant efficacy.
- Improving cell survival during cryopreservation is critical for various applications.
Purpose of the Study:
- To compare four cryoprotectant variants modified with egg yolk and lecithin.
- To evaluate custom-developed yolk emulsions against commercial options.
Main Methods:
- Cryoprotectant effectiveness assessed via cytotoxicity and vital screening.
- Sperm motility index determined to evaluate cryoprotective effects.
- Analytical model developed for cholesterol concentration estimation.
Main Results:
- Custom egg yolk modifications demonstrated superior performance.
- Higher fractions of living cells, motility, and membrane integrity observed.
- Cholesterol concentration model aids in stabilizing cellular membranes.
Conclusions:
- Phosphatidylcholine contributes to egg yolk's cryoprotective action but is not the sole factor.
- The cryoprotective effect of egg yolk on plasma membranes is multifaceted.
- No single component fully explains the cryoprotective benefits of egg yolk.
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