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Updated: Jan 24, 2026
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Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions
Published on: January 20, 2022
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Characterisation of the Dynamic Interactions between Complex N-Glycans and Human CD22
Cristina Di Carluccio1, Enrique Crisman2, Yoshiyuki Manabe3,4
1Dipartimento di Scienze Chimiche, Complesso Universitario Monte Sant'Angelo, Università di Napoli Federico II, Via Cintia 4, 80126, Napoli, Italy.
Chembiochem : a European Journal of Chemical Biology
|May 17, 2019
Summary
Human CD22 (Siglec-2) protein binds to sialylated glycans, regulating autoimmune responses. This study reveals molecular details of this interaction, aiding therapeutic development for B-cell malignancies and autoimmune diseases.
Area of Science:
- Biochemistry
- Immunology
- Structural Biology
Background:
- CD22 (Siglec-2) is a B-cell inhibitory receptor that recognizes sialylated glycans.
- It plays a crucial role in dampening autoimmune responses and maintaining self-tolerance.
- Dysregulation of CD22 function is implicated in autoimmune diseases and B-cell malignancies.
Purpose of the Study:
- To characterize the dynamic recognition of complex-type N-glycans by human CD22 (h-CD22).
- To gain molecular insights into the binding interactions and conformational dynamics.
- To understand CD22 homo-oligomerization on B-cell surfaces.
Main Methods:
- Nuclear Magnetic Resonance (NMR) spectroscopy
- Computational modeling
- Biophysical assays
Main Results:
- Detailed molecular insights into the binding mode of sialoglycans with h-CD22.
- Identified the crucial role of sialic acid and galactose moieties in recognition.
- Elucidated the conformational behavior of N-glycans bound to CD22.
- Dissected the formation of CD22 homo-oligomers on B-cell surfaces.
Conclusions:
- The study provides a comprehensive understanding of h-CD22-glycan interactions.
- Findings highlight key molecular determinants for CD22 recognition.
- Results pave the way for developing novel therapeutics targeting h-CD22 for autoimmune diseases and B-cell cancers.
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