Related Experiment Video
Updated: Jan 24, 2026
05:08
A TNBS-Induced Rodent Model to Study the Pathogenic Role of Mechanical Stress in Crohn's Disease
Published on: March 1, 2022
4.5K
Urine Proteome Changes in a TNBS-Induced Colitis Rat Model.
Weiwei Qin1,2, Lujun Li1, Ting Wang1
1Department of Biochemistry and Molecular Biology, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing Normal University, Beijing, 100875, China.
Proteomics. Clinical Applications
|May 17, 2019
Summary
Urine proteins can serve as biomarkers for inflammatory bowel disease (IBD), including Crohn's disease (CD). This study identified 77 urinary proteins altered in a colitis rat model, offering new diagnostic clues.
Area of Science:
- Proteomics
- Biomarker Discovery
- Gastroenterology
Background:
- Urine is a valuable source for biomarker research, reflecting systemic health beyond kidney function.
- Urine proteomic analysis for inflammatory bowel disease (IBD) remains underexplored.
Purpose of the Study:
- To investigate urinary protein changes in a rat model of inflammatory bowel disease (IBD) to identify potential biomarkers for Crohn's disease (CD).
Main Methods:
- Utilized a trinitrobenzene sulfonic acid (TNBS)-induced colitis rat model.
- Employed label-free and TMT-labeled quantitative proteomics for urine sample analysis.
- Validated candidate proteins using parallel reaction monitoring (PRM) targeted proteomics.
Main Results:
- Identified 77 significantly altered urinary proteins in colitis rats compared to controls.
- Highlighted carbonic anhydrase 1, ribonuclease pancreatic gamma type, and rBAT as gastrointestinally enriched.
- Confirmed known IBD-associated proteins and discovered novel candidates, including carbonic anhydrase 1, neutrophil collagenase, and neutrophil gelatinase-associated lipocalin.
Conclusions:
- The study provides crucial insights into urinary biomarkers for inflammatory bowel disease (IBD) and Crohn's disease (CD).
- Identified proteins offer potential for non-invasive diagnostic and monitoring strategies.
More Related Videos
Related Concept Videos
01:32Filtration and Urine Formation
53.4K
The function of the kidneys is to filter, reabsorb, secrete, and excrete. Every day the kidneys filter nearly 180 liters of blood, initially removing water and solutes but ultimately returning nearly all filtrates into circulation with the help of osmoregulatory hormones. This process removes wastes and toxins but is also crucial to maintain water and electrolyte levels. Most of these functions are performed by the tiny but numerous nephrons contained within the kidneys.
53.4K
01:26Urine Studies II: Urine Culture and Sensitivity Test
971
A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
971
01:13Induced-fit Model
89.0K
Most chemical reactions in cells require enzymes—biological catalysts that speed up the reaction without being consumed or permanently changed. They reduce the activation energy needed to convert the reactants into products. Enzymes are proteins, that usually work by binding to a substrate—a reactant molecule that they act upon.
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...
89.0K
01:24Physiology of Urine Formation
12.3K
Urine formation is an essential function of the human body. It plays a critical role in maintaining homeostasis by regulating the volume and composition of body fluids. The kidneys, the primary organs involved in this process, filter blood to remove waste products and excess substances, ultimately producing urine.
Glomerular Filtration
The first stage in urine formation is glomerular filtration. Each kidney contains approximately 1 million nephrons, the functional units of filtration, with a...
Glomerular Filtration
The first stage in urine formation is glomerular filtration. Each kidney contains approximately 1 million nephrons, the functional units of filtration, with a...
12.3K
01:23Formation of Concentrated Urine
3.2K
There is a gradient of solutes in the interstitial fluid from the renal cortex through the medulla, known as the medullary osmotic gradient. The juxtamedullary nephrons establish and maintain this gradient using countercurrent mechanisms with loops extending deep into the medulla. These nephrons also use countercurrent mechanisms to regulate urine volume and concentration. The interaction between the descending and ascending limbs of the nephron loop creates an osmotic gradient through...
3.2K
01:29Urine Studies I: Urinalysis
1.2K
Urinalysis is a widely used diagnostic test that analyzes urine's physical, chemical, and microscopic characteristics. Healthcare providers use it to detect and monitor various health conditions, including renal disease, urinary tract infections (UTIs), diabetes, and metabolic or systemic disorders.Components of UrinalysisUrinalysis consists of three primary components: physical, chemical, and microscopic examination. Each provides unique insights into the urine sample and, by extension, the...
1.2K