Related Experiment Video
Updated: Jan 24, 2026

A TNBS-Induced Rodent Model to Study the Pathogenic Role of Mechanical Stress in Crohn's Disease
Published on: March 1, 2022
Urine Proteome Changes in a TNBS-Induced Colitis Rat Model
Weiwei Qin1,2, Lujun Li1, Ting Wang1
1Department of Biochemistry and Molecular Biology, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing Normal University, Beijing, 100875, China.
Purpose:
Urine is an important resource for biomarker research. Urine proteins reflect not only renal diseases but also changes in other organs in the body. However, urine has rarely been used to reflect on inflammatory bowel disease.
Experimental Design:
In the present study, a trinitrobenzene sulfonic acid (TNBS)-induced colitis rat model is used to mimic the human inflammatory bowel disease Crohn's disease (CD). Urine samples are analyzed by label-free and TMT-labeled proteomic quantitative methods.
Results:
77 urinary proteins are significantly changed in the colitis rats compared with that in the controls. These proteins are further validated by parallel reaction monitoring (PRM) targeted proteomic quantitative methods. Based on the human protein tissue atlas, carbonic anhydrase 1, ribonuclease pancreatic gamma type, and neutral and basic amino acid transport protein rBAT are highly enriched in the gastrointestinal tract. Among the nine PRM-validated proteins, carbonic anhydrase 1, neutrophil collagenase, and neutrophil gelatinase associated lipocalin were previously reported as IBD-associated proteins (all exhibiting consistent trends with the observation herein), whereas the others are newly discovered by this study.
Conclusions And Clinical Relevance:
The results provide valuable clues for future study of urine biomarker of inflammatory bowel disease and Crohn's disease.
More Related Videos
Related Concept Videos
Filtration and Urine Formation
Urine Studies II: Urine Culture and Sensitivity Test
Induced-fit Model
Enzymes exhibit substrate specificity, meaning that they can only bind to certain substrates. This is mainly determined by the shape and chemical...
Physiology of Urine Formation
Glomerular Filtration
The first stage in urine formation is glomerular filtration. Each kidney contains approximately 1 million nephrons, the functional units of filtration, with a...
Formation of Concentrated Urine
Urine Studies I: Urinalysis

