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Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications
Published on: February 23, 2017
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Dextran-Coated Zinc-Doped Hydroxyapatite for Biomedical Applications
Daniela Predoi1, Simona Liliana Iconaru2, Mihai Valentin Predoi3
1National Institute of Materials Physics, Atomistilor Street, No. 405A, P.O. Box MG 07, 077125 Magurele, Romania. dpredoi@gmail.com.
Polymers
|May 18, 2019
Summary
Dextran-coated zinc-doped hydroxyapatite (ZnHApD) nanoparticles show promising non-toxic effects on human cells and potent antibacterial activity against various microorganisms. ZnHApD effectively inhibits bacterial growth, with notable sensitivity observed in Porphyromonas gingivalis.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Microbiology
Background:
- Hydroxyapatite is a key biomaterial, and doping it with zinc (ZnHApD) can enhance its properties.
- Developing stable and effective nanomaterials is crucial for biomedical applications.
- Understanding the biocompatibility and antimicrobial potential of novel materials is essential.
Purpose of the Study:
- To synthesize and characterize dextran-coated zinc-doped hydroxyapatite (ZnHApD) nanoparticles.
- To evaluate the stability, biocompatibility, and antibacterial efficacy of ZnHApD.
- To investigate the dose-dependent effects of ZnHApD on human cells and various bacterial strains.
Main Methods:
- Sol-gel synthesis for ZnHApD nanoparticle preparation.
- Ultrasonic measurements for stability analysis.
- X-ray diffraction (XRD), Transmission Electron Microscopy (TEM), and Scanning Electron Microscopy (SEM) for material characterization.
- HEK-293 cell culture assays for cytotoxicity and cell growth.
- Antibacterial assays against multiple microbial strains.
Main Results:
- ZnHApD nanoparticles were successfully synthesized with an ellipsoidal shape and narrow size distribution.
- Ultrasonic analysis confirmed the stability of ZnHApD in aqueous solutions.
- XRD showed no impurities in the synthesized material.
- ZnHApD exhibited non-toxic activity against HEK-293 cells at all tested concentrations.
- ZnHApD demonstrated significant antibacterial activity against all tested microorganisms.
- Porphyromonas gingivalis ATCC 33277 was the most sensitive strain, showing inhibition at 0.075 μg/mL.
Conclusions:
- Dextran-coated zinc-doped hydroxyapatite (ZnHApD) is a stable nanomaterial with excellent biocompatibility.
- ZnHApD possesses broad-spectrum antibacterial properties, making it a potential candidate for antimicrobial applications.
- The material's efficacy varies depending on the bacterial strain, with P. gingivalis being particularly susceptible.
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