Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

7.7K
The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
Graded and Abrupt Responses
Some signaling systems generate...
7.7K
CNS Stimulants: Cocaine, Amphetamines and Cannabinoids01:24

CNS Stimulants: Cocaine, Amphetamines and Cannabinoids

819
CNS stimulants, such as cocaine, amphetamines, and cannabinoids, have varying structures and mechanisms of action that lead to different therapeutic effects and side effects. Cocaine, with its molecular formula C17H21NO4, is a tropane alkaloid and a tertiary amino compound. It has two chemical forms: the hydrochloride salt and the "freebase." The former is in powder form, while the latter involves removing the hydrochloride salt to create a form that can be smoked. Cocaine exerts its...
819
Chemotherapy-Induced Nausea and Vomiting: Cannabinoids01:21

Chemotherapy-Induced Nausea and Vomiting: Cannabinoids

716
Tetrahydrocannabinol (THC) is a phytocannabinoid that primarily interacts with the CB1 receptor, a type of G protein-coupled receptor (GPCR) predominantly in and around the chemoreceptor trigger zone (CTZ) and emetic center. THC also blocks the serotonin receptor activity in the dorsal vagal complex (DVC) by inhibiting serotonin release. THC exerts its anti-emetic effects through these interactions, which are beneficial for patients undergoing chemotherapy.
Two synthetic agonists of THC,...
716
Diversity of Archaea I01:30

Diversity of Archaea I

567
Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...
567
Diversity of Archaea II01:24

Diversity of Archaea II

468
Archaea, one of the three domains of life, exhibit remarkable diversity and adaptability, thriving in both extreme and moderate environments. Historically, most identified archaea have been classified into two major phyla: Euryarchaeota and Crenarchaeota. However, recent molecular studies have expanded this classification to include three additional phyla: Thaumarchaeota, Nanoarchaeota, and Korarchaeota, each exhibiting unique characteristics and ecological roles.Thaumarchaeota: Mesophiles...
468
Diversity of Protists I01:15

Diversity of Protists I

908
Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
908

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence and Tradition in Dialogue: Biological Sex Variability in Phytomedicine Research as a Foundation for Safety, Efficacy, and Robust Evidence Standards.

Medicines (Basel, Switzerland)·2026
Same author

OPTILATER: optimal long-term survival after cancer - a cross-sectional study protocol for a quantitative survey on the care situation of long-term cancer survivors in Germany.

BMC cancer·2025
Same author

Computational analysis of therapeutic potential for simplified Piper. spp- derived medicinal mixtures in anxiety, sleep, pain and seizure.

bioRxiv : the preprint server for biology·2025
Same author

A pathway to next-generation mast cell stabilizers identified through the novel Phytomedical Analytics for Research Optimization at Scale data platform.

bioRxiv : the preprint server for biology·2025
Same author

WITHDRAWN: Gender Dysphoria Preceding Intersex Recognition Demonstrates a Biophysiological Basis for Sex Identity.

Research square·2025
Same author

Antimicrobial usage and stewardship in a hospice setting.

The Journal of hospital infection·2024

Related Experiment Video

Updated: Jan 24, 2026

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

8.4K

Diverse TRPV1 responses to cannabinoids.

J Starkus1, C Jansen1, L M N Shimoda1

  • 1a Laboratory of Immunology and Signal Transduction , Chaminade University , Honolulu , HI , USA.

Channels (Austin, Tex.)
|May 18, 2019
PubMed
Summary

Cannabinoids show potential for pain relief by interacting with specific ion channels like TRPV1. Different cannabinoids activate these channels uniquely, suggesting targeted pain management strategies.

Keywords:
TRP channelscalciumcannabinoidsoxidationpain

More Related Videos

Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies
05:25

Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies

Published on: December 29, 2021

2.9K
Murine Distal Colostomy, A Novel Model of Diversion Colitis in C57BL/6 Mice
08:20

Murine Distal Colostomy, A Novel Model of Diversion Colitis in C57BL/6 Mice

Published on: July 12, 2018

13.9K

Related Experiment Videos

Last Updated: Jan 24, 2026

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis
11:53

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

8.4K
Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies
05:25

Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies

Published on: December 29, 2021

2.9K
Murine Distal Colostomy, A Novel Model of Diversion Colitis in C57BL/6 Mice
08:20

Murine Distal Colostomy, A Novel Model of Diversion Colitis in C57BL/6 Mice

Published on: July 12, 2018

13.9K

Area of Science:

  • Pharmacology
  • Neuroscience
  • Pain Research

Background:

  • Cannabinoid compounds are recognized for their analgesic properties.
  • Medicinal cannabis use is reported effective for pain management, potentially reducing opioid reliance.
  • Specific cannabinoids target nociceptive ion channels involved in pain signaling.

Purpose of the Study:

  • To investigate and compare the effects of various cannabinoids on the physiology of the TRPV1 ion channel.
  • To analyze the differential activation and kinetics of cannabinoids on nociceptive ion channels.

Main Methods:

  • Electrophysiological recordings to assess cannabinoid interactions with TRPV1.
  • Investigation of calcium dependence on cannabinoid-TRPV1 activation.
  • Comparative analysis of cannabinoid activity on TRPV1, TRPV2, TRPM8, and TRPA1 channels.

Main Results:

  • Cannabinoids induced varied activation and inactivation kinetics for TRPV1.
  • Cannabinoid activation of TRPV1 was significantly influenced by intracellular and extracellular calcium concentrations.
  • Unlike capsaicin, cannabinoids did not appear to induce a highly permeant, pore-dilated TRPV1 state.
  • Cannabinoids differentially activated other nociceptive channels, including TRPV2, TRPM8, and TRPA1.

Conclusions:

  • Cannabinoid modulation of TRPV1 exhibits distinct kinetic and calcium-dependent properties.
  • Cannabinoids display differential selectivity across various nociceptive ion channels.
  • Rational design of novel pain therapeutics may be achievable by utilizing single or combined cannabinoids based on their specific channel interactions.