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Long noncoding RNA DDX11-AS1 epigenetically represses LATS2 by interacting with EZH2 and DNMT1 in hepatocellular

Yong Li1, Wenlong Zhuang2, Maosheng Huang2

  • 1Department of Liver Transplantation Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 201129, China; Department of Hepatobiliary Surgery, Suqian First Hospital, Suqian, Jiangsu, 223800, China.

Biochemical and Biophysical Research Communications
|May 18, 2019
PubMed

Insights

Long noncoding RNA DDX11-AS1 is highly expressed in hepatocellular carcinoma (HCC), promoting tumor growth and invasion by suppressing LATS2. This suggests DDX11-AS1 is a potential oncogene and therapeutic target for HCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long noncoding RNAs (lncRNAs) are implicated in hepatocellular carcinoma (HCC) progression.
  • The specific role of DDX11-AS1 in HCC pathogenesis remains largely unknown.

Purpose of the Study:

  • To investigate the biological function and clinical significance of DDX11-AS1 in hepatocellular carcinoma.
  • To elucidate the molecular mechanism underlying DDX11-AS1's role in HCC.

Main Methods:

  • Quantitative real-time PCR and western blotting to assess gene expression.
  • Cell proliferation, migration, invasion, and cell cycle assays.
  • RNA immunoprecipitation (RIP) and chromatin immunoprecipitation (ChIP) assays.
  • In vivo tumor formation assays.

Main Results:

  • DDX11-AS1 expression is significantly upregulated in HCC tissues and cell lines, correlating with poor patient survival.
  • DDX11-AS1 silencing inhibits HCC cell proliferation, migration, and invasion, while its overexpression promotes these processes.
  • DDX11-AS1 interacts with EZH2 and DNMT1 to suppress LATS2 expression, thereby promoting HCC growth and invasion.
  • DDX11-AS1 promotes tumor formation in vivo, and LATS2 expression is negatively correlated with DDX11-AS1 levels in tumor tissues.

Conclusions:

  • DDX11-AS1 acts as a novel oncogene in hepatocarcinogenesis by repressing LATS2.
  • DDX11-AS1 represents a potential therapeutic target for hepatocellular carcinoma treatment.
Keywords:
DDX11-AS1EpigeneticHepatocellular carcinomaLATS2

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