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Profile analysis reveals transfer RNA fragments involved in mesangial cells proliferation

Xiaoyu Lu1, Xianyi Zhu1, Minyi Yu1

  • 1Department of Pediatric Nephrology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210003, China.

Biochemical and Biophysical Research Communications
|May 18, 2019
PubMed

Insights

Transfer RNA fragments (tRFs) were investigated as biomarkers for mesangial cell proliferation in glomerulonephritis. Two specific tRFs, tDR-000064 and tDR-000103, were found to be down-regulated and correlated with mesangial cell proliferation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Nephrology

Background:

  • Mesangial cell (MC) proliferation is a key factor in glomerulonephritis pathogenesis.
  • Identifying reliable biomarkers for MC proliferation is crucial for understanding and treating kidney diseases.

Purpose of the Study:

  • To investigate the role of transfer RNA fragments (tRFs) as potential biomarkers for mesangial cell proliferation.
  • To analyze the differential expression of tRFs in proliferating mesangial cells.

Main Methods:

  • Mesangial cells were treated with transforming growth factor-beta 1 (TGF-β1) to induce proliferation.
  • High-throughput sequencing was employed to analyze tRF expression profiles.
  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used for validation.
  • Receiver operating characteristic (ROC) curve analysis was performed to assess diagnostic potential.
  • Gene Ontology (GO) and pathway analysis were conducted.

Main Results:

  • Two tRFs, tDR-000064 and tDR-000103, were significantly down-regulated in TGF-β1-treated (proliferating) mesangial cells compared to normal mesangial cells.
  • tDR-000064 and tDR-000103 expression levels were correlated with mesangial cell proliferation.
  • GO and pathway analysis indicated involvement of these tRFs in MCs and TGF-β1 receptor-mediated signaling.

Conclusions:

  • tDR-000064 and tDR-000103 are potential biomarkers for mesangial cell proliferation.
  • These findings contribute to understanding the molecular mechanisms of glomerulonephritis.
  • Further research into tRFs could offer novel therapeutic targets for kidney diseases.
Keywords:
Mesangial cellsProliferationTransfer RNA fragments

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