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Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing
05:17

Detection of Targetable Alterations in Non-small Cell Lung Cancer using Next-generation Sequencing

Published on: October 10, 2025

367

Altered expression of microRNA-365 is related to the occurrence and development of non-small-cell lung cancer by

Qian Han1, Peng Cheng1, Hongjie Yang1

  • 1Department of Radiotherapy, Henan Provincial People's Hospital, Zhengzhou, Henan, People's Republic of China.

Journal of Cellular Physiology
|May 18, 2019
PubMed

Insights

Altered microRNA-365 (miR-365) expression is linked to non-small-cell lung cancer (NSCLC) development. This study shows miR-365 inhibits TRIM25, impacting NSCLC progression and potentially offering new therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Non-small-cell lung cancer (NSCLC) remains a leading cause of cancer mortality globally.
  • MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
  • Dysregulation of specific miRNAs, including miR-365, has been implicated in various cancers.

Purpose of the Study:

  • To investigate the association between altered microRNA-365 (miR-365) expression and the initiation/development of non-small-cell lung cancer (NSCLC).
  • To determine if miR-365 targets TRIM25 expression in NSCLC.
  • To explore the functional impact of miR-365 on NSCLC cell behavior.

Main Methods:

  • Expression analysis of miR-365 and TRIM25 in NSCLC tissues and cell lines.
  • Correlation analysis between miR-365/TRIM25 expression and NSCLC clinicopathological features.
  • Luciferase activity assay to confirm direct targeting of TRIM25 by miR-365.
  • In vitro functional assays (cell viability, cell cycle, apoptosis) using miR-365 mimics and inhibitors in NSCLC cells.

Main Results:

  • Downregulation of miR-365 and upregulation of TRIM25 were observed in NSCLC tissues.
  • TRIM25 was confirmed as a direct target gene of miR-365.
  • miR-365 and TRIM25 expression levels correlated significantly with NSCLC pathological classification, differentiation, TNM stage, and lymph node metastasis.
  • miR-365 mimics suppressed NSCLC cell viability and proliferation, induced apoptosis, and inhibited TRIM25 expression.

Conclusions:

  • Altered miR-365 expression is closely associated with the occurrence and development of NSCLC.
  • miR-365 exerts its tumor-suppressive effects in NSCLC by inhibiting TRIM25 expression.
  • Targeting the miR-365/TRIM25 axis presents a potential therapeutic strategy for NSCLC.
Keywords:
TRIM25apoptosismicroRNA-365non-small-cell lung cancerproliferation

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