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Glucagon-like peptides activate hepatic gluconeogenesis.

T P Mommsen, P C Andrews, E M Plisetskaya

    FEBS Letters
    |July 13, 1987
    PubMed
    Summary

    Piscine glucagon-like peptides (GLPs) potently activate gluconeogenesis in fish liver cells, surpassing human GLP-1 and GLP-2 effects. These fish hormones stimulate glucose production without typical cyclic nucleotide signaling pathways.

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    Metabolic actions of glucagon-family hormones in liver.

    Fish physiology and biochemistry·2013

    Area of Science:

    • Comparative endocrinology
    • Fish physiology
    • Metabolic regulation

    Background:

    • Glucagon-like peptides (GLPs) are crucial metabolic regulators.
    • Their role in fish gluconeogenesis, particularly piscine GLPs, is not fully elucidated.
    • Understanding GLP function in fish is vital for comparative physiology.

    Purpose of the Study:

    • To investigate the effect of piscine and human GLPs on gluconeogenesis in fish hepatocytes.
    • To compare the potency of piscine GLPs versus human GLPs and glucagon.
    • To explore the intracellular signaling mechanisms involved.

    Main Methods:

    • Isolated trout and salmon hepatocytes were used.
    • Cells were treated with piscine GLPs, human GLP-1, GLP-2, and glucagon at 3.5 nM.
    • Flux through gluconeogenesis and substrate oxidation were measured.
    • Intracellular messengers (cAMP, cGMP) were assessed.

    Main Results:

    • Piscine GLPs significantly stimulated gluconeogenesis (1.1-1.9-fold) in trout and salmon hepatocytes.
    • Human GLP-1 and GLP-2 showed weaker activation compared to piscine counterparts.
    • Piscine GLPs were more potent activators than glucagon at equimolar concentrations.
    • Minor increases in substrate oxidation were observed during peak activation.
    • cAMP and cGMP were not identified as intracellular messengers.

    Conclusions:

    • Piscine GLPs are potent stimulators of hepatic gluconeogenesis in fish.
    • Fish GLPs exhibit distinct signaling pathways, independent of cAMP/cGMP.
    • These findings highlight the specialized roles of GLPs in fish metabolic control.

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