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Plaquing of Herpes Simplex Viruses
Published on: November 5, 2021
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Herpes simplex virus resistant to acyclovir: A single-centre experience from the Czech Republic
Miroslav Fajfr1, Lenka Pliskova2, Radka Bolehovská2
1Institute of Clinical Microbiology, University Hospital in Hradec Kralove, Czech Republic; Charles University in Prague, Faculty of Medicine in Hradec Kralove, Czech Republic.
Journal of Global Antimicrobial Resistance
|May 18, 2019
Summary
Herpes simplex virus (HSV) antiviral resistance mutations were identified in Czech patients, including specific mutations in UL30 for HSV1 and UL23/UL30 for HSV2. Further studies are needed to confirm these findings in the Czech population.
Area of Science:
- Virology
- Molecular Biology
- Antimicrobial Resistance
Background:
- Herpes simplex virus (HSV) infections are common globally.
- Increasing use of antiviral medications has led to rising antiviral resistance.
- Limited data exists on HSV antiviral resistance in the Czech Republic.
Purpose of the Study:
- To investigate the prevalence of antiviral resistance mutations in HSV isolates from the Czech Republic.
- To identify specific mutations associated with antiviral failure in HSV1 and HSV2.
Main Methods:
- Retrospective analysis of 22 HSV isolates (15 HSV1, 7 HSV2) from patients with suspected antiviral failure (2009-2016).
- DNA sequencing of genes associated with antiviral resistance (UL23 and UL30).
- Utilized ABI PRISM 3500xL Genetic Analyzer for sequence analysis.
Main Results:
- No resistance mutations were found in the UL23 gene of HSV1 isolates.
- HSV1 isolates showed resistant mutation D672N in the UL30 gene (7/15 isolates).
- HSV2 isolates exhibited acyclovir resistance mutation M183stop in UL23 (1/7 isolates) and resistant mutation E678G in UL30 (6/7 isolates).
Conclusions:
- This study confirms the presence of antiviral resistance mutations in HSV within the Czech population.
- Specific mutations conferring resistance to antivirals were identified in both HSV1 and HSV2.
- Further investigation with a larger sample size is recommended to draw more definitive conclusions.
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