Evaluation of Angiogenesis Assays
Related Experiment Video
Updated: Jan 24, 2026

Rat Mesentery Angiogenesis Assay
Published on: June 18, 2011
Zachary I Stryker1, Mehdi Rajabi2, Paul J Davis3,4
1Department of Health, Division of Environmental Health Sciences, Albany, NY 12237, USA. zachary.stryker@health.ny.gov.
Abstract:
Angiogenesis assays allow for the evaluation of pro- or anti-angiogenic activity of endogenous or exogenous factors (stimulus or inhibitors) through investigation of their pro-or anti- proliferative, migratory, and tube formation effects on endothelial cells. To model the process of angiogenesis and the effects of biomolecules on that process, both in vitro and in vivo methods are currently used. In general, in vitro methods monitor specific stages in the angiogenesis process and are used for early evaluations, while in vivo methods more accurately simulate the living microenvironment to provide more pertinent information. We review here the current state of angiogenesis assays as well as their mechanisms, advantages, and limitations.
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