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Circular RNA ACVR2A suppresses bladder cancer cells proliferation and metastasis through miR-626/EYA4 axis

Wei Dong1,2, Junming Bi1,2, Hongwei Liu1,2

  • 1Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107th Yanjiangxi Road, Guangzhou, China.

Molecular Cancer
|May 19, 2019
PubMed
Abstract

Insights

Circular RNA ACVR2A (circACVR2A) acts as a tumor suppressor in bladder cancer by inhibiting cell proliferation and metastasis. Its down-regulation correlates with aggressive disease, suggesting circACVR2A is a potential prognostic biomarker and therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in human cancer development, often functioning as microRNA (miRNA) sponges.
  • The specific role and molecular mechanisms of circRNAs in bladder cancer remain largely uncharacterized.

Purpose of the Study:

  • To investigate the expression profile and functional role of circACVR2A in bladder cancer.
  • To elucidate the molecular mechanism by which circACVR2A regulates bladder cancer progression.

Main Methods:

  • circACVR2A expression was quantified using RNA-sequencing and qRT-PCR.
  • In vitro and in vivo assays assessed circACVR2A's role in bladder cancer cell proliferation and metastasis.
  • miRNA sponge activity was confirmed via pull-down assays, miRNA capture, dual-luciferase reporter assays, and FISH.

Main Results:

  • circACVR2A expression was significantly lower in bladder cancer tissues and cell lines compared to normal controls.
  • Down-regulation of circACVR2A correlated with aggressive clinicopathological features and poorer overall survival.
  • circACVR2A suppressed bladder cancer cell proliferation, migration, and invasion by directly interacting with miR-626 and regulating EYA4 expression.

Conclusions:

  • circACVR2A acts as a tumor suppressor in bladder cancer, inhibiting cell proliferation and metastasis via the miR-626/EYA4 axis.
  • circACVR2A represents a promising prognostic biomarker and potential therapeutic target for bladder cancer.
Keywords:
Bladder cancerEYA4MetastasisProliferationcircACVR2AmiRNA-626

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