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Pseudosecretion of Escherichia coli chloramphenicol acetyltransferase by Bacillus subtilis
Journal of Bacteriology
|July 1, 1987
Abstract:
Bacillus subtilis harboring the vector 25RBSII secrets an Escherichia coli-derived chloramphenicol acetyltransferase into culture supernatants. The secreted enzyme lacks 18 amino acids; these are removed externally rather than during secretion.