Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Crossing Over01:34

Crossing Over

169.6K
Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...
169.6K
Crossing Over01:30

Crossing Over

6.3K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
6.3K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.9K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.9K
Monohybrid Crosses01:20

Monohybrid Crosses

238.8K
Overview
238.8K
Elastin is Responsible for Tissue Elasticity01:12

Elastin is Responsible for Tissue Elasticity

3.1K
Elastic fiber contains the protein elastin along with lesser amounts of other proteins and glycoproteins. The main property of elastin is that it will return to its original shape after being stretched or compressed. Elastic fibers are prominent in elastic tissues found in skin and the elastic ligaments of the vertebral column.
Ligaments and tendons are made of dense regular connective tissue, but in ligaments not all fibers are parallel. Dense regular elastic tissue contains elastin fibers and...
3.1K
Cross-Sectional Research01:50

Cross-Sectional Research

12.4K
In cross-sectional research, a researcher compares multiple segments of the population at the same time. If they were interested in people's dietary habits, the researcher might directly compare different groups of people by age. Instead of following a group of people for 20 years to see how their dietary habits changed from decade to decade, the researcher would study a group of 20-year-old individuals and compare them to a group of 30-year-old individuals and a group of 40-year-old...
12.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring mechanisms of scar-free skin wound healing in adult zebrafish in comparison to mouse.

PLoS genetics·2026
Same author

Systemic clinical-grade recombinant decorin reduces disease burden and fibrosis in advanced recessive dystrophic epidermolysis bullosa.

Molecular therapy. Advances·2026
Same author

Isolation and Characterization of Extracellular Vesicles Derived from Mango Fruits.

International journal of molecular sciences·2025
Same author

Characterization of Small Extracellular Vesicles Isolated from <i>Aurelia aurita</i>.

Biology·2025
Same author

A standardized in vitro bioengineered skin for penetrating wound modeling.

In vitro models·2025
Same author

Advances in Extracellular Matrix-Associated Diagnostics and Therapeutics.

Journal of clinical medicine·2025

Related Experiment Video

Updated: Jan 24, 2026

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
09:57

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

Published on: December 23, 2016

9.3K

A comprehensive map of human elastin cross-linking during elastogenesis.

Tobias Hedtke1,2, Christoph U Schräder3, Andrea Heinz3,4

  • 1Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Halle (Saale), Germany.

The FEBS Journal
|May 19, 2019
PubMed
Summary

Elastin cross-linking, crucial for tissue elasticity, is more complex than previously thought. This study reveals stochastic, varied cross-linking patterns within human tropoelastin, involving multiple lysine residues and domains.

Keywords:
desmosineelastic fibersextracellular matrixlysyl oxidasemass spectrometry

More Related Videos

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia
12:25

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia

Published on: January 6, 2018

8.3K
Photo-Induced Cross-Linking of Unmodified Proteins PICUP Applied to Amyloidogenic Peptides
08:40

Photo-Induced Cross-Linking of Unmodified Proteins PICUP Applied to Amyloidogenic Peptides

Published on: January 12, 2009

21.9K

Related Experiment Videos

Last Updated: Jan 24, 2026

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
09:57

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

Published on: December 23, 2016

9.3K
Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia
12:25

Second Harmonic Generation Signals in Rabbit Sclera As a Tool for Evaluation of Therapeutic Tissue Cross-linking TXL for Myopia

Published on: January 6, 2018

8.3K
Photo-Induced Cross-Linking of Unmodified Proteins PICUP Applied to Amyloidogenic Peptides
08:40

Photo-Induced Cross-Linking of Unmodified Proteins PICUP Applied to Amyloidogenic Peptides

Published on: January 12, 2009

21.9K

Area of Science:

  • Biochemistry
  • Structural Biology
  • Extracellular Matrix Research

Background:

  • Elastin provides elasticity to tissues like skin and blood vessels.
  • Its unique properties stem from covalent cross-linking of tropoelastin monomers.
  • The precise mechanisms of elastin cross-linking remain poorly understood.

Purpose of the Study:

  • To investigate the molecular structure and cross-linking patterns of human elastin.
  • To identify different types of cross-links and involved residues.
  • To clarify the contribution of specific domains to elastin cross-linking.

Main Methods:

  • Purification of human elastin.
  • Enzymatic cleavage into soluble peptides.
  • High-resolution mass spectrometry and amino acid analysis.
  • Identification of cross-linked peptides and post-translational modifications.

Main Results:

  • Revealed complex and heterogeneous molecular interconnections in elastin.
  • Identified instances where lysine residues participate in multiple cross-link types or remain unmodified.
  • Demonstrated that Lys-Pro and Lys-Ala domains form both inter- and intra-domain cross-links.
  • Elucidated desmosine-containing peptides, including a novel three-domain cross-link structure.

Conclusions:

  • Elastin cross-linking occurs in a stochastic manner within and between tropoelastin units.
  • The findings challenge previous assumptions about desmosine cross-link formation.
  • Provides new insights into the structural basis of tissue elasticity.