Characterization and differentiation of CD51⁺ Stem Leydig cells in adult mouse testes

It was reported previously that adult mouse stem Leydig cells (SLCs) express CD51 (integrin α-chain V). However, it is still unclear whether all CD51⁺ cells are SLCs. In the present study, we found that CD51⁺ cells can be classified into two sub-groups, a weakly-staining group (CD51⁺) and a strongly-staining group (CD51⁺⁺). The CD51⁺ cells expressed common SLC marker genes, including Nestin, Pdgfra and Coup-tf2, while CD51⁺⁺ cells did not express these genes. Instead, they expressed macrophage markers, such as F4/80, Cd115 and Tnfa. When these cells were induced to differentiate in vitro, the CD51⁺ cells, but not CD51⁺⁺ cells, formed Leydig cells. Overall, our results showed that although SLCs expressed CD51, not all CD51-expressing cells are SLCs. The cells that expressed high levels of CD51 are actually macrophages.