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Updated: Jan 24, 2026

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface
Published on: July 21, 2016
Trophoblast function is altered by decidual factors in gestational-dependant manner
E M Menkhorst1, M Van Sinderen1, J Correia2
1Centre for Reproductive Health, Hudson Institute of Medical Research, Clayton, VIC, 3186, Australia; Department of Molecular and Translational Medicine, Monash University, Clayton, VIC, 3800, Australia.
Abstract:
Inadequate implantation and placentation is associated with miscarriage and placental insufficiency. The decidual environment is thought to regulate trophoblast invasion, however this is poorly defined in humans. We aimed to determine the effect of decidualization on trophoblast function. In vitro decidualized primary human endometrial stromal cells (HESC) significantly enhanced first-trimester extravillous trophoblast (EVT) (6-8-weeks gestation) adhesion, outgrowth/invasion. In EVTs from 10 to 12-weeks gestation this effect was absent (adhesion, invasion) or reversed (outgrowth). HESC conditioned media had no effect on trophoblast MMP9 production/activity. Decidualization regulated EVT function in a gestational-dependent manner. This study highlights the importance of trophoblast-decidual synchrony.
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