Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bioequivalence Data: Statistical Interpretation01:16

Bioequivalence Data: Statistical Interpretation

202
Body:The statistical interpretation of bioequivalence data is a significant aspect of pharmaceutical research. Bioequivalence refers to the absence of any significant difference in the rate and extent to which the active ingredient in pharmaceutical products becomes available at the site of drug action when administered at the same molar dose under similar conditions. This helps determine if different drug products have similar absorption rates, ensuring their interchangeability.Statistical...
202
Fundamental Attribution Error01:14

Fundamental Attribution Error

13.7K
According to some social psychologists, people tend to overemphasize internal factors as explanations—or attributions—for the behavior of other people. They tend to assume that the behavior of another person is a trait of that person, and to underestimate the power of the situation on the behavior of others. They tend to fail to recognize when the behavior of another is due to situational variables, and thus to the person’s state. This erroneous assumption is...
13.7K
Interference and Diffraction02:18

Interference and Diffraction

51.9K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
51.9K
RNA Interference01:23

RNA Interference

27.9K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.9K
Timing and Consequences on Behavior01:08

Timing and Consequences on Behavior

370
In operant conditioning, the timing of reinforcement is crucial. For animals like rats and cats, immediate reinforcement (within a few seconds) is much more effective than delayed reinforcement. For example, a food reward for a rat needs to follow within 30 seconds of pressing a bar to be effective. 
Humans, however, can respond to delayed reinforcers. We often make decisions between immediate small rewards and delayed larger rewards. This ability to delay gratification is a significant...
370
Systematic Error: Methodological and Sampling Errors01:15

Systematic Error: Methodological and Sampling Errors

9.9K
In the case of systematic errors, the sources can be identified, and the errors can be subsequently minimized by addressing these sources. According to the source, systematic errors can be divided into sampling, instrumental, methodological, and personal errors.
Sampling errors originate from improper sampling methods or the wrong sample population. These errors can be minimized by refining the sampling strategy. Defective instruments or faulty calibrations are the sources of instrumental...
9.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NRF2 as a guardian of health: from an ancient survival pathway to a modern therapeutic target.

Redox biology·2026
Same author

Endophytic Fungi as a Promising Source of Bioactive Compounds for Wound Healing: A Systematic Review.

Microorganisms·2026
Same author

Expression, Solubilization, and Refolding Recovery of a Novel l‑Asparaginase-Arginase 1 Chimera from <i>E. coli</i> Inclusion Bodies.

ACS omega·2026
Same author

Tailored high-cell-density fed-batch cultivation and induction strategies for optimized production of a low-immunogenic L-asparaginase mutant in Escherichia coli.

Enzyme and microbial technology·2026
Same author

Proteomic profiling of a Sporobolomyces yeast reveals global responses to UV-B-induced oxidative stress.

PloS one·2026
Same author

Cysteine Pegylation of a Mutant L-asparaginase Affords Enhanced Activity and Thermostability. A Comparative Study Against N-terminal Conjugation.

Applied biochemistry and biotechnology·2026

Related Experiment Video

Updated: Jan 24, 2026

Heat Tolerance Assays Using the Drosophila Activity Monitor System: A Guide to an Executable Application for Data Analysis
05:05

Heat Tolerance Assays Using the Drosophila Activity Monitor System: A Guide to an Executable Application for Data Analysis

Published on: December 13, 2024

1.0K

Interferences that impact measuring optimal L-asparaginase activity and consequent errors interpreting these data.

Marcela Medeiros de Freitas1, Paula Monteiro Souza1, Kellen Cruvinel1

  • 1Natural Products Laboratory, Health Sciences School, University of Brasília, Brasília, 70910-900, Brazil.

Applied Microbiology and Biotechnology
|May 20, 2019
PubMed
Summary

L-asparaginase, an enzyme crucial for leukemia treatment and food safety, may have its activity overestimated. This review highlights potential inaccuracies in current assay methods due to endogenous ammonia interference.

Keywords:
Enzyme assayInterferencesL-AsparaginaseNessler method

More Related Videos

Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source
12:19

Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source

Published on: April 4, 2017

8.8K
Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research
08:12

Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research

Published on: February 16, 2024

15.5K

Related Experiment Videos

Last Updated: Jan 24, 2026

Heat Tolerance Assays Using the Drosophila Activity Monitor System: A Guide to an Executable Application for Data Analysis
05:05

Heat Tolerance Assays Using the Drosophila Activity Monitor System: A Guide to an Executable Application for Data Analysis

Published on: December 13, 2024

1.0K
Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source
12:19

Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source

Published on: April 4, 2017

8.8K
Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research
08:12

Author Spotlight: Exploring Light-Driven Chemical Reactions and Energy-Harnessing Devices in Photochemical Research

Published on: February 16, 2024

15.5K

Area of Science:

  • Enzymology
  • Biotechnology
  • Microbiology

Background:

  • L-asparaginase is a vital enzyme used in acute lymphoblastic leukemia (ALL) treatment and food processing to prevent acrylamide formation.
  • Microbial sources are extensively studied for L-asparaginase production.
  • Assessing enzyme activity is critical for both clinical and industrial applications.

Purpose of the Study:

  • To review microbial sources of L-asparaginase.
  • To evaluate culture media for optimizing enzyme expression.
  • To assess common assay methods for L-asparaginase activity.

Main Methods:

  • Literature search of PubMed and Web of Science databases up to January 22, 2018.
  • Evaluation of articles based on enzyme source, culture media nitrogen, sample type, and assay method.
  • Focus on bacterial L-asparaginase and the Nessler method for activity assessment.

Main Results:

  • Bacterial L-asparaginase is the most frequently studied source.
  • The Nessler method, measuring ammonia, is commonly used for activity assays.
  • Endogenous ammonia from microbial fermentation can interfere with Nessler reagent, potentially inflating activity measurements.

Conclusions:

  • Current estimates of L-asparaginase activity using the Nessler method may be overestimated.
  • The interference of fermentation-derived ammonia is often not accounted for in reported studies.
  • Further research is needed to refine assay methodologies for accurate L-asparaginase activity determination.