Related Experiment Video

Updated: Jan 24, 2026

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
09:52

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

11.0K

The application of next-generation sequencing in diagnosing invasive pulmonary aspergillosis: three case reports

Bin-Chan He1, Lu-Lu Liu1, Bi-Lin Chen1

  • 1Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Nanjing University Nanjing 210002, China.

American Journal of Translational Research
|May 21, 2019
PubMed

Insights

Next-generation sequencing (NGS) aids in diagnosing invasive pulmonary aspergillosis (IPA), a serious lung infection. This rapid method detected Aspergillus fumigatus in patients, including those without typical risk factors like neutropenia.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Genomics

Background:

  • Invasive pulmonary aspergillosis (IPA) presents diagnostic challenges due to difficulties in obtaining microbiological evidence.
  • Current microbiological methods for IPA diagnosis are often slow and have low sensitivity.

Observation:

  • This study presents three cases of IPA, with two patients having a history of chronic obstructive pulmonary disease (COPD) and asthma.
  • Next-generation sequencing (NGS) was employed to detect the Aspergillus fumigatus gene in bronchoalveolar lavage fluid.

Findings:

  • NGS successfully identified Aspergillus fumigatus in all three IPA cases, demonstrating its utility in pathogen detection.
  • The study highlights that IPA can occur in patients without neutropenia, broadening the clinical suspicion criteria.

Implications:

  • NGS offers a faster and more accurate alternative for diagnosing IPA compared to traditional methods.
  • These findings suggest a need to consider IPA in a wider range of patients, including non-neutropenic individuals.
Keywords:
Next generation sequencingdiagnostic methodinvasive pulmonary aspergillosislung infection

More Related Videos

Establishment of a Minimally Invasive Rat Model of Pulmonary Embolism Using Autologous Blood Clots08:02

Establishment of a Minimally Invasive Rat Model of Pulmonary Embolism Using Autologous Blood Clots

Published on: October 25, 2024

797
Collection and Extraction of Saliva DNA for Next Generation Sequencing06:58

Collection and Extraction of Saliva DNA for Next Generation Sequencing

Published on: August 27, 2014

40.0K

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
98.0K
Diagnosing Acidosis and Alkalosis01:24

Diagnosing Acidosis and Alkalosis

Diagnosing acid-base imbalances involves systematically analyzing arterial blood samples, focusing on three key measurements: pH, bicarbonate (HCO3−) concentration, and carbon dioxide partial pressure (PCO2). This analysis follows a four-step process that helps identify the imbalance's underlying cause and nature.
First, the pH level is assessed to determine whether the blood pH is normal (7.35–7.45), low (acidosis), or high (alkalosis).
Next, the PCO2  and...
1.1K
Data Reporting and Recording01:24

Data Reporting and Recording

Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
5.4K
Types of Reports I: Hands-off Report01:25

Types of Reports I: Hands-off Report

A hand-off report, also known as a change-of-shift report, is a crucial nursing process that ensures the smooth transition of patient care responsibilities between nursing staff.
Following are the key components and categories of hand-off reports:
Purpose and Process:
1.5K
Types of Reports II: Incident or Occurrence Report01:21

Types of Reports II: Incident or Occurrence Report

An Incident or Occurrence Report in a healthcare setting is a crucial document used to record any unexpected occurrence that may or may not have affected a patient, employee, or visitor. Such reports are critical to improving patient safety and include all details leading up to and including the event.
Purposes:
In the healthcare industry, reports play a crucial role in documenting incidents within an agency. The primary objective of these reports is to ensure patient safety, uphold the...
1.2K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.6K