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Updated: Jan 24, 2026

Bacterial Peptide Display for the Selection of Novel Biotinylating Enzymes
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Ectoine hydroxylase displays selective trans-3-hydroxylation activity towards L-proline

Ryotaro Hara1, Takeyuki Nishikawa2, Takuya Okuhara2

  • 1Research Institute for Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo, 169-8555, Japan.

Applied Microbiology and Biotechnology
|May 21, 2019
PubMed
Summary

This study developed a biocatalytic process for trans-3-Hydroxyproline (trans-3-Hyp) production using engineered E. coli. Deleting a specific gene enhanced trans-3-Hyp yield, benefiting pharmaceutical synthesis.

Keywords:
2-Oxoglutarate-dependent dioxygenaseEctoine hydroxylaseHydroxylationHydroxyprolinetrans-3-Hydroxy-L-proline

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