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Total flavonoids from the Carya cathayensis Sarg. leaves inhibit HUVEC senescence through the miR-34a/SIRT1 pathway

Yan Guo1, Liwan Xing1, Nipi Chen1

  • 1College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.

Journal of Cellular Biochemistry
|May 21, 2019
PubMed

Insights

Total flavonoids (TFs) inhibit endothelial cell senescence, a key factor in vascular aging and cardiovascular disease. This effect is mediated by the miR-34a/Sirtuin1 pathway, offering potential therapeutic insights.

Area of Science:

  • Vascular Biology
  • Cellular Senescence
  • Molecular Mechanisms

Background:

  • Aging is linked to vascular dysfunction, with endothelial cell senescence being a major contributor to cardiovascular diseases.
  • Advanced age is a nonmodifiable risk factor for cardiovascular conditions.

Purpose of the Study:

  • To investigate the impact of total flavonoids (TFs) on human umbilical vein endothelial cell (HUVEC) senescence.
  • To elucidate the underlying molecular mechanisms involving the miR-34a/SIRT1 pathway.

Main Methods:

  • A HUVEC senescence model was induced using angiotensin II.
  • Senescence markers (SA-β-gal, p53, p21, cell cycle distribution) were assessed.
  • Quantitative PCR and Western blot analyzed miR-34a and SIRT1 expression, with and without TF and miR-34a inhibitor treatments.

Main Results:

  • TFs reduced SA-β-gal positive cells and G0/G1 arrest, increasing S phase.
  • TFs decreased miR-34a expression and increased SIRT1 expression.
  • Co-treatment with TFs and a miR-34a inhibitor further reduced SA-β-gal and miR-34a, while increasing SIRT1.

Conclusions:

  • Total flavonoids effectively inhibit HUVEC senescence.
  • The anti-senescence mechanism involves the regulation of the miR-34a/Sirtuin1 pathway.

Keywords:
HUVECSIRT1miR-34asenescencetotal flavonoids from the Carya cathayensis Sarg. leaves

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