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Grape seeds proanthocyanidin extract ameliorates Ehrlich solid tumor induced renal tissue and DNA damage in mice

Mabrouk Attia Abd Eldaim1, Ehab Tousson2, Ibrahim El Tantawy El Sayed3

  • 1Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Menoufia University, Shebin Elkom, Menoufia, Egypt.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|May 21, 2019
PubMed

Insights

Grape seed proanthocyanidins extract (GSPE) protects kidneys from Ehrlich solid tumor (EST) induced injury. GSPE treatment normalized kidney function and reduced DNA damage and protein expression in renal tissues.

Area of Science:

  • Biochemistry
  • Toxicology
  • Pharmacology

Background:

  • Ehrlich solid tumor (EST) can induce significant renal injury.
  • Assessing the protective effects of natural compounds against drug-induced or disease-induced organ damage is crucial.

Purpose of the Study:

  • To evaluate the protective effect of grape seed proanthocyanidins extract (GSPE) against Ehrlich solid tumor (EST) induced renal injury.
  • To investigate the impact of GSPE on DNA fragmentation and P53, PCNA, and ki67 protein expression in renal tissue.

Main Methods:

  • 50 female mice were divided into five groups: control, GSPE-treated, EST-induced, EST + GSPE (simultaneous treatment), and EST + GSPE (delayed treatment).
  • Renal injury was assessed by measuring serum urea, creatinine, potassium, sodium, and calcium levels.
  • Renal tissue and DNA integrity were evaluated, along with the expression of P53, PCNA, and ki67 proteins.

Main Results:

  • EST significantly elevated serum urea, creatinine, potassium, and chloride, while decreasing sodium and calcium.
  • EST induced renal tissue damage, DNA fragmentation, and increased P53, PCNA, and ki67 expression.
  • GSPE treatment normalized serum electrolyte and kidney function markers, improved renal tissue structure, and reduced DNA damage and protein overexpression.

Conclusions:

  • Grape seed proanthocyanidins extract (GSPE) demonstrates significant nephron protective properties against Ehrlich solid tumor (EST) induced renal injury.
  • GSPE mitigates renal damage by reducing DNA fragmentation and modulating the expression of key proteins involved in cell proliferation and apoptosis.
Keywords:
Ehrlich solid tumorGrape seed proanthocyanidins extractKi67LiverP53PCNA

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