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Biohydrogen and polyhydroxyalkanoate production from original hydrolyzed polyacrylamide-containing wastewater
Lanmei Zhao1, Dong Han2, Zichao Yin1
1Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education/Institute for Advanced Ocean Study, Ocean University of China, Qingdao 266100, China; College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China.
Abstract:
This work aimed to study biohydrogen (H2) and polyhydroxyalkanoate (PHA) production from original hydrolyzed polyacrylamide (HPAM)-containing wastewater. NH4+-N from HPAM hydrolysis was removed efficiently through short-cut nitrification and anoxic ammonia oxidation (anammox). Carbon/Nitrogen (C/N) ratios of effluent reached 51-97, and TOC decreased only 2%-4%, providing potential for subsequent H2 and PHA production. The maximum yields of H2 (0.833 mL·mg-1substrate) and Volatile Fatty Acid (VFA) (465 mg·L-1) occurred at influent C/N ratio of 51. Substrate removal increased linearly with the activities of dehydrogenase and hydrogenase (R2 ≥ 0.990), and H2 yield rose exponentially with enzyme activities (R2 ≥ 0.989). The maximum PHA yield (54.2% VSS) occurred at the 42nd hour and influent C/N ratio of 97. PHA yield was positively correlated with substrate uptake. The change of H2-producing, PHA-accumulating and HPAM-degradating bacteria indicated that those functional microorganisms had synergistic effects on H2 production and substrate uptake, as well as PHA accumulation and substrate uptake.
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