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Updated: Jan 24, 2026

Cell Co-culture Patterning Using Aqueous Two-phase Systems
Published on: March 26, 2013
Dynamic phases, stratification, laning, and pattern formation for driven bidisperse disk systems in the presence of
D McDermott1,2, Y Yang1,3, C J Olson Reichhardt1
1Theoretical Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Abstract:
Using numerical simulations, we examine the dynamics of driven two-dimensional bidisperse disks flowing over quenched disorder. The system exhibits a series of distinct dynamical phases as a function of applied driving force and packing fraction, including a phase-separated state as well as a smectic state with liquid-like or polycrystalline features. At low driving forces, we find a clogged phase with an isotropic density distribution, while at intermediate driving forces the disks separate into bands of high and low densities with either liquid-like or polycrystalline structure in the high-density bands. In addition to the density phase separation, we find that in some cases there is a fractionation of the disk species, particularly when the disk size ratio is large. The species phase-separated regimes form a variety of patterns such as large disks separated by chains of smaller disks. Our results show that the formation of laning states can be enhanced by tuning the ratio of disk radius of the two species, due to the clumping of small disks in the interstitial regions between the large disks. This system could be experimentally realized using sterically interacting colloidal particles suspended in a viscous fluid driven over random pinning arrays or granular matter suspended in fluid moving over a random landscape.
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